D201大孔离子交换树脂固定糖化酶的研究  

Study on Immobilization of Glycosylase on D201 Macroporous Ion-exchange Resin

作　　者：余志英 巫锦雄[1] 王小兰[1] 刘文敏 YU Zhi-ying;WU Jin-xiong;WANG Xiao-lan;LIU Wen-min(School of Life Sciences,Guangzhou University,Guangdong Guangzhou 410005,China)

机构地区：[1]广州大学生命科学学院,广东广州410005

出　　处：《广州化工》2021年第1期32-37,共6页GuangZhou Chemical Industry

基　　金：广州大学2019年度校级示范性实验项目(SJ201904);2020年挑战杯广州大学院级培育项目。

摘　　要：在单因素实验基础上,本文以D201大孔离子交换树脂为载体进行糖化酶的固定,以酶活回收率为评价指标,分析了固定化时间、料液比(酶与载体的比例)、给酶量等因素在糖化酶固定化中的影响。通过响应面法分析得到固定化最佳工艺为:固化时间27 min,料液比1:1,给酶量1800 U/g。此条件下的酶活回收率为60.66%,响应面优化结果验证良好。此外,通过稳定性检测发现:固定化糖化酶在常温下的半衰期为5天,低温4℃时保存较佳,操作稳定性较差。On the basis of single factor test,D201 macroporous ion-exchange resin was used as carrier to immobilize glucoamylase,and the recovery rate of enzyme activity was used as evaluation index,the effects of immobilization time,ratio of material to liquid(the ratio of enzyme to carrier)and amount of enzyme on the immobilization of glucoamylase were analyzed.Response surface methodology(RSM)was used to determine the optimum conditions of immobilization.The optimum conditions were as follows:the time of immobilization was 27 min,the ratio of material to liquid was 1:1,and the enzyme dosage was 1800 U/g.Under these conditions,the recovery of enzyme activity was 60.66%,and the result of response surface optimization was good.In addition,the half-life of immobilized saccharifying enzyme was 5 days at room temperature,it was better preserved at 4℃,and the stability of operation was worse.

关 键 词：糖化酶 固定化 离子交换树脂 响应面法 

分 类 号：TS241[轻工技术与工程—制糖工程]