同位素稀释-气相色谱-串联质谱法测定熟肉中多组分β-受体激动剂  

Determination of multi-residueβ-agonists in cooked meat by isotope dilution-gas chromatography-tandem mass spectrometry

作　　者：曹忠波[1] 曹歌 张媛媛[1] 刘晓晶[1] 华正罡[1] CAO Zhong-Bo;CAO Ge;ZHANG Yuan-Yuan;LIU Xiao-Jing;HUA Zheng-Gang(Center for Disease Control and Prevention of Liaoning,Shenyang 110005,China;College of Food Science Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]辽宁省疾病预防控制中心,沈阳110005 [2]沈阳农业大学食品学院,沈阳110866

出　　处：《食品安全质量检测学报》2020年第24期9140-9146,共7页Journal of Food Safety and Quality

摘　　要：目的建立气相色谱-串联质谱法(gas chromatography-tandem mass spectrometry,GC-MS/MS)检测熟肉制品中多种β-受体激动剂残留量的方法。方法样品经β-葡萄糖醛苷酶/芳基硫酸酯酶酶解,酶解液通过MCX固相萃取柱浓缩净化,后用5%氨化乙酸乙酯洗脱,经过双三甲基硅基三氟乙酰胺+1%(φ)三甲基氯硅烷衍生,用GC-MS/MS测定,同位素内标法定量。结果β-受体激动剂在0.05~1 mg/L浓度范围内,线性关系良好,相关系数r>0.9994,回收率为80.3%~109.5%,精密度(RSD)在10%以内。结论该方法精密度好、灵敏度高,可简便、准确地测定熟肉中多种β-受体激动剂的残留量。Objective To establish a method for determination of residues ofβ-agonists in cooked meat products by gas chromatography-tandem mass spectrometry(GC-MS/MS).Methods The sample was hydrolyzed byβ-glucuronidase/arylsulfatase,concentrated and purified by MCX solid phase extraction column,eluted with 5%ammoniated ethyl acetate,and derivatized with bis(trimethylsilyl)-trifluoroacetamide+1%(φ)trimethylchlorosilane,and then determined by GC-MS/MS,and quantified by isotope internal standard method.Results The linear relationship ofβ-agonist was good in the range of 0.05-1 mg/L,the correlation coefficient r>0.9994,the recoveries were 80.3%-109.5%,and the relative standard deviations(RSDs)were 2.1%-7.6%.Conclusions The method is accurate and sensitive,and can be used for the determination of residualβ-agonists in cooked meat.

关 键 词：β-受体激动剂 熟肉 同位素稀释 气相色谱-串联质谱法 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程] O657.63[轻工技术与工程—食品科学与工程]