响应面法优化海参蒸煮废液皂苷的提取工艺  

Optimization of extraction process of saponins from sea cucumber cooking waste liquid by response surface method

作　　者：衣丹 王炜 洪旭光 张朝晖 YI Dan;WANG Wei;HONG Xu-Guang;ZHANG Zhao-Hui(First Institute of Oceanography,Ministry of Natural Resources,Qingdao 266061,China)

机构地区：[1]自然资源部第一海洋研究所,青岛266061

出　　处：《食品安全质量检测学报》2020年第24期9528-9532,共5页Journal of Food Safety and Quality

基　　金：青岛市科技开发项目(A201203)。

摘　　要：目的优化海参蒸煮废液中皂苷的提取工艺。方法通过单因素试验,研究了乙醇体积分数、提取温度、料液比和提取时间4个因素对皂苷提取率的影响,并通过Box-Benhnken中心组合试验及响应面分析法对提取条件进行优化。结果最优提取工艺条件为:乙醇体积分数64%,提取温度56℃,料液比1:2 g/mL,海参皂苷的提取率为1.68%。结论经过工艺优化,海参皂苷的提取率明显提高,为海参废液的资源化利用提供依据。Objective To optimize the extraction process of saponins from sea cucumber cooking waste liquid.Methods Through single factor experiment,the influence of ethanol volume fraction,extraction temperature,rate of material and solution and extraction time on the extraction yield of saponin was studied,and the extraction conditions were optimized by Box-Benhnken central combination experiment and response surface analysis.Results The optimal extraction conditions were 64%of ethanol volume fraction,extraction temperature 56℃,rate of material and solution 1:2 g/mL.Under these conditions,the degree of extraction yield was 1.68%.Conclusion After optimization of the processes,the extraction rate of sea cucumber saponins is significantly improved,which provides the basis for the resource utilization of sea cucumber waste liquid.

关 键 词：海参 蒸煮废液 海参皂苷 响应面分析法 

分 类 号：X792[环境科学与工程—环境工程] TQ464.3[化学工程—制药化工]