山楂粗糖蛋白对高脂小鼠降脂及抗氧化作用的初步研究  

Effect of crude hawthorn glycoprotein on hypolipidemic and antioxidant activity in high-fat mice

作　　者：高雪婧 罗思奕 汤凯洁[1] 罗秋水[1] GAO Xuejing;LUO Siyi;TANG Kaijie;LUO Qiushui(School of Food Science and Technology,Jiangxi Agricultural University,Nanchang 330045,China)

机构地区：[1]江西农业大学食品学院,江西南昌330045

出　　处：《食品与发酵工业》2021年第1期138-142,共5页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31760488);江西农业大学大学生创新创业训练项目(202010410231)。

摘　　要：采用水提醇沉法提取有效成分,Sevage法去除游离蛋白,DEAE-52纤维素层析及Sephadex G-75纯化制备山楂粗糖蛋白,并选择昆明(KM)小鼠为研究对象,随机分为空白对照组、高脂模型组、山楂粗糖蛋白低、中、高剂量组。8周后检测小鼠血清总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)含量;检测肝脏过氧化氢酶(catalase,CAT)、总超氧化物歧化酶(total superoxide dismutase,T-SOD)活性,总抗氧化能力(total antioxidant capacity,T-AOC)和丙二醛(malonaldehyde,MDA)含量。结果表明,与空白对照组相比,高脂模型组的TC、TG、MDA水平升高,HDL-C、CAT、T-SOD、T-AOC降低,且均极显著差异(P <0. 01);与高脂模型组相比,山楂粗糖蛋白组的TC、TG、MDA水平降低(P <0. 05),HDL-C、CAT、T-SOD、T-AOC含量升高(P <0. 05),量效关系显著,由此可知,山楂粗糖蛋白对高脂小鼠具有良好的降脂作用和抗氧化活性。The hypolipidemic and antioxidant activity of crude glycoprotein from hawthorn in high-fat mice were investigated in the paper.The active ingredients were extracted by water extraction and alcohol precipitation,the free protein was removed by Sevage method,DEAE-52 cellulose chromatography and SephadexG-75 purification were used to prepare crude hawthorn glycoprotein.KM mice were selected as the research objects,and randomly divided into blank control group,high-fat model group,hawthorn glycoprotein low,medium and high dose groups.After 8 weeks of gavage,the content of total cholesterol(TC),triglyceride(TG)and high density lipoprotein cholesterol(HDL-C)in serum were measured,the activities of catalase(CAT),total superoxide dismutase(T-SOD),total antioxidant capacity(T-AOC)and the content of malonaldehyde(MDA)in liver were measured.Results showed that compared with the blank control group,the levels of TC,TG and MDA in the high-fat model group increased,while HDL-C,CAT,T-SOD and T-AOC decreased,all the differences were significant(P<0.01).Compared with the high-fat model group,the content of TC,TG and MDA in the hawthorn glycoprotein group decreased(P<0.05),and the content of HDL-C and the activities of CAT,T-SOD and T-AOC increased(P<0.05).The dose-effect relationship was significant,from this we can find that the crude glycoprotein from hawthorn had a better effect on hypolipidemic and antioxidant activity in high-fat mice.

关 键 词：山楂 粗糖蛋白 高脂小鼠 降脂 抗氧化 

分 类 号：TS201.4[轻工技术与工程—食品科学]