In vitro immunomodulation of magnesium on monocytic cell toward anti-inflammatory macrophages  

作　　者：Lei Sun Xiaoyu Li Menghan Xu Fenghe Yang Wei Wang Xufeng Niu 

机构地区：[1]Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education,School of Biological Science and Medical Engineering,Beihang University,No.37 XueYuan Road,Haidian District,Beijing 100083,China [2]Beijing Advanced Innovation Center for Biomedical Engineering,Beihang University,No.37 XueYuan Road,Haidian District,Beijing 100083,China [3]Department of Immunology,School of Basic Medical Sciences,NHC Key Laboratory of Medical Immunology,Peking University,No.38 XueYuan Road,Haidian District,Beijing 100191,China

出　　处：《Regenerative Biomaterials》2020年第4期391-401,共11页再生生物材料（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(11872097,31872735);Beijing Natural Science Foundation(L182017);the Fundamental Research Funds for the Central Universities(YWF-19-BJ-J-234);the 111 Project(B13003);the International Joint Research Center of Aerospace Biotechnology and Medical Engineering,Ministry of Science and Technology of China.

摘　　要：Biodegradable magnesium(Mg)has shown great potential advantages over current bone fixation devices and vascular scaffold technologies;however,there are few reports on the immunomodulation of corrosive Mg products,the micron-sized Mg particles(MgMPs).Human monocytic leukemia cell line THP-1 was set as the in vitro cell model to estimate the immunomodulation of MgMPs on cell proliferation,apoptosis,polarization and inflammatory reaction.Our results indicated highconcentration of Mg^2+ demoted the proliferation of the THP-1 cells and,especially,THP-1-derived macrophages,which was a potential factor that could affect cell function,but meanwhile,cell apoptosis was almost not affected by Mg^2+.In particular,the inflammation regulatory effects of MgMPs were investigated.Macrophages exposed to Mg^2+ exhibited down-regulated expressions of M1 subtype markers and secretions of pro-inflammatory cytokines,up-regulated expression of M2 subtype marker and secretion of anti-inflammatory cytokine.These results indicated Mg^2+ could convert macrophages from M0 to M2 phenotype,and the bioeffects of MgMPs on human inflammatory cells were most likely due to the Mg^2+-induced NF-jB activation reduction.Together,our results proved Mg^2+ could be used as a new anti-inflammatory agent to suppress inflammation in clinical applications,which may provide new ideas for studying the immunomodulation of Mg-based implants on human immune system.

关 键 词：IMMUNOMODULATION MAGNESIUM THP-1 MACROPHAGE polarization 

分 类 号：R318[医药卫生—生物医学工程]