苦荞NAC转录因子FtNAC17的鉴定及表达分析  

Identification and Expression of NAC Transcription Factor FtNAC17 in Tartary Buckwheat

作　　者：荣玉萍 唐彬 李鹏[1] 章洁琼 陈庆富[1] 朱丽伟[1] 邓娇[1] 黄娟[1] RONG Yu-ping;TANG Bin;LI Peng;ZHANG Jie-qiong;CHEN Qing-fu;ZHU Li-wei;DENG Jiao;HUANG Juan(Research Center of Buckwheat Industry Technology,Guizhou Normal University,Guiyang 550001;Guizhou Agricultural Technology Extension Station,Guiyang 550001)

机构地区：[1]贵州师范大学荞麦产业技术研究中心,贵阳550001 [2]贵州省农作物技术推广总站,贵阳550001

出　　处：《生物技术通报》2021年第1期174-181,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31760419,31760430);贵州省科技计划(黔科合基础[2016]1107;黔科合成果[2019]4252号;黔科合平台人才[2017]5726-19号;黔科合LH字[2017]7364号);贵州师范大学资助博士科研项目(11904/0516031)。

摘　　要：植物特有的NAC转录因子参与植物生长发育和逆境响应。研究苦荞中NAC转录因子在非生物胁迫中的应答,为阐明基因功能提供理论依据。以苦荞为试验材料,克隆一个NAC家族基因,对其进行生物信息学分析。通过荧光定量PCR技术检测该基因在干旱、低温、盐、茉莉酸甲酯、脱落酸和赤霉素胁迫时的响应情况。该基因编码272个氨基酸,将其命名为FtNAC17,GenBank登录号为MT641452。基因结构分析表明,FtNAC17由2个外显子和1个内含子组成。氨基酸序列多重比对和进化关系分析表明,FtNAC17蛋白与拟南芥ANAC002亲缘关系最近,属于NAC转录因子家族中同一亚组。FtNAC17对干旱、低温、盐等非生物胁迫均有不同程度的响应。Plant specific NAC transcription factors are involved in plant growth and development and stress response.To study the response of NAC transcription factors in tartary buckwheat to abiotic stress would provide theoretical basis for elucidating gene function.A NAC family’s gene from tartary buckwheat was cloned and then analyzed by bioinformatics.The response of the gene to drought,low temperature,salt,methyl jasmonate,abscisic acid and gibberellin was detected by fluorescence quantitative PCR.The gene encodes 272 amino acids,which is named FtNAC17 and GenBank accession number is MT641452.The analysis of gene structure showed that FtNAC17 gene was composed of two exons and one intron.Multiple alignment of amino acid sequences and phylogenetic analysis revealed that FtNAC17 protein was the closest relative to ANAC002 in Arabidopsis thaliana,belonging to the same subgroup of NAC transcription factor family.FtNAC17 presented varied responses to drought,low temperature and salt abiotic stress.

关 键 词：苦荞 FtNAC17 克隆 表达 非生物胁迫 

分 类 号：Q943.2[生物学—植物学] S517[农业科学—作物学]