核桃内种皮黄酮的提取工艺优化及抗氧化性研究  

Optimization of Extraction Technology and Antioxidant Activity of Flavonoids from Walnut Kernel Pellicle

作　　者：时羽杰 肖徐 李晶晶 糜加轩 万雪琴[1] SHI Yujie;XIAO Xu;LI Jingjing;MI Jiaxuan;WAN Xueqin(College of Forestry,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学林学院,四川成都611130

出　　处：《食品工业科技》2021年第2期192-198,209,共8页Science and Technology of Food Industry

基　　金：四川省育种攻关项目(2016NYZ0035)。

摘　　要：为了优化核桃内种皮黄酮的超声提取工艺并对其进行抗氧化性和主要成分进行初步探究,在单因素实验基础上采用响应面分析法研究乙醇浓度、料液比、提取温度对黄酮得率的影响。同时采用水杨酸比色法和邻苯三酚自氧化测定提取物的抗氧化能力并测定其主要化学组成。结果表明,当乙醇浓度59%,料液比1∶52 g/mL,提取温度41℃,超声功率500 W,提取时间30 min时,内种皮黄酮得率为15.58%。抗氧化活性试验表明,在10~50μg/mL浓度范围内,内种皮黄酮对·OH和·O-2的最大清除率分别达到73.25%和65.27%。采用UPLC-MS/MS平台分析,共鉴定到119种黄酮类化合物,包括52种黄酮醇,18种黄烷醇类,18种黄酮,8种二氢黄酮,5种花青素,5种异黄酮,3种二氢黄酮醇,2种查尔酮。因此,优化后超声提取核桃内种皮黄酮效果较好,具有较强的抗氧化作用并对其主要成分进行了鉴定,为核桃资源的开发利用奠定基础。In order to optimize the ultrasonic extraction technology of flavonoids from walnut kernel pellicle and its antioxidant activity and main components,the response surface methodology(RSM)was used to study the effects of ethanol concentration,liquid-material ratio and extraction temperature on the yield of flavonoids based on the single experiment.The antioxidant capacity of the extract was determined by salicylic acid colorimetry and pyrogallol autoxidation,and the main chemical composition were identified by UPLC-MS/MS.The results showed that when the concentration of ethanol was 59%,the liquid-material ratio was 1∶52 g/mL,the extraction temperature was 41℃,the ultrasonic power was 500 W,and the extraction time was 30 min,the yield of flavonoids in kernel pellicle was 15.58%.The antioxidant activity test showed that in the concentration range of 10~50μg/mL,the maximum scavenging rates of·OH and·O-2by endotestal flavonoids were 73.25%and 65.27%,respectively.A total of 119 flavonoid compound were identified by UPLC-MS/MS platform analysis,including 52 flavonols,18 flavonols,18 flavanoids,8 dihydroflavonoids,5 anthocyanins,5 isoflavones,3 dihydroflavonols and 2 chalcone.Therefore,the optimized ultrasonic extraction of walnut kernel pellicle flavonoids has a good effect with a strong antioxidant effect and identified its main components,which lays a foundation for the development and utilization of walnut resources.

关 键 词：核桃 内种皮 黄酮 响应面法 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]