大蒜素对卵巢癌细胞侵袭转移的抑制作用及机制  

Inhibitory effect and mechanism of diallyl trisulfide on invasion and metastasis of ovarian cancer cells

作　　者：万慧芳[1] 梁笑倾[2] 刘卓琦[3] 杨晓红[3] 张霞丽[4] 李华[4] 万福生[3] WAN Huifang;LIANG Xiaoxiang;LIU Zhuoqi;YANG Xiaohong;ZHANG Xiaoli;LI Hua;WAN Fusheng(Nanchang University Medical Experimental Teaching Center,Nanchang 330006,China;Nanchang University Obstetrics and Gynecology Department of the Fourth Affiliated Hospital,Nanchang 330006,China;Nanchang University Department of Biochemistry and Molecular Biology,Basic Medical College,Nanchang 330006,China;Nanchang University Experimental Animal Science Center,Nanchang 330006,China)

机构地区：[1]南昌大学医学实验教学中心,江西南昌330006 [2]南昌大学第四附属医院妇产科,江西南昌330006 [3]南昌大学基础医学院生物化学与分子生物学教研室,江西南昌330006 [4]南昌大学实验动物科学中心,江西南昌330006

出　　处：《南昌大学学报（理科版）》2020年第5期463-469,共7页Journal of Nanchang University(Natural Science)

基　　金：江西省自然科学基金资助项目(20171BAB215059)。

摘　　要：探讨大蒜素(DT)对人卵巢癌细胞侵袭转移的影响及机制。用不同浓度DT处理卵巢癌SKOV3,ES-2细胞,CCK8检测细胞增殖活性,Transwell小室检测细胞迁移与侵袭能力,Western blot检测细胞蛋白表达。结果显示,DT(终浓度为2.5~20μg·mL-1)对人卵巢癌细胞增殖和侵袭迁移有不同程度的抑制作用(P<0.05),且呈一定的量效关系;同时,DT能增加上皮-间质转化(EMT)标志蛋白E-cadherin表达,下调E-cadherin,Vimentin蛋白及基质金属蛋白酶(MMPs)表达;激活AKT/GSK-3β通路及降低β-catenin蛋白含量。结果表明DT具有抑制人卵巢癌细胞EMT与侵袭转移的作用,其机制可能与AKT/GSK-3β/β-catenin信号通路有关。To investigate the effect and mechanism of diallyl trisulfide(DT)on invasion and metastasis of human ovarian cancer cells,the ovarian cancer SKOV3,ES-2 cells were treated with DT at different concentrations.The CCK8 detected the proliferative activity of cells and the transwell assay detected the ability of cell migration and invasion.Also,the western blot was used to detect protein expression in cells.The results showed that DT(final concentration:2.5-20μg·mL-1)inhibited the proliferation,invasion and migration of ovarian cancer cells to varying degrees(P<0.05).Also,a certain dose-effect relationship was observed.Meanwhile,DT increased the expression of E-cadherin,a marker of epithelial-mesenchymal transformation(EMT),and down-regulated the expression of E-cadherin,Vimentin and matrix metalloproteinases(MMPs).It also activated the AKT/GSK-3βpathway and reduced theβ-catenin protein.The results showed that DT can inhibit EMT,invasion and metastasis of ovarian cancer cells,and the mechanism might be related to AKT/GSK-3β/β-catenin signaling pathway.

关 键 词：卵巢癌 大蒜素 侵袭转移 上皮-间质转化 信号通路 

分 类 号：R737.31[医药卫生—肿瘤]