Detection of fucosylated haptoglobin using the 10-7G antibody as a biomarker for evaluating endoscopic remission in ulcerative colitis  

作　　者：Kei Motooka Koichi Morishita Nami Ito Shinichiro Shinzaki Taku Tashiro Satoshi Nojima Kayoko Shimizu Mutsuhiro Date Natsumi Sakata Momoko Yamada Shinji Takamatsu Yoshihiro Kamada Hideki Iijima Tsunekazu Mizushima Eiichi Morii Tetsuo Takehara Eiji Miyoshi 

机构地区：[1]Department of Molecular Biochemistry and Clinical Investigation,Osaka University Graduate School of Medicine,Suita 565-0871,Osaka,Japan [2]Department of Gastroenterology and Hepatology,Osaka University Graduate School of Medicine,Suita 565-0871,Osaka,Japan [3]Department of Pathology,Osaka University Graduate School of Medicine,Suita 565-0871,Osaka,Japan [4]FUJIFILM Wako Pure Chemical Corporation,Amagasaki 661-0963,Hyogo,Japan [5]Department of Gastroenterological Surgery,Osaka University Graduate School of Medicine,Suita 565-0871,Osaka,Japan

出　　处：《World Journal of Gastroenterology》2021年第2期162-175,共14页世界胃肠病学杂志（英文版）

基　　金：Ministry of Education,Culture,Sports,Science and Technology of Japan,No.19H03562;FUJIFILM Wako Pure Chemical Corporation,No.J770701626.

摘　　要：BACKGROUND Inflammatory bowel disease(IBD)is a chronic,relapsing inflammation of the digestive tract.Although fecal and serum biomarkers have been extremely important and supportive for monitoring of IBD,their low sensitivity and high variability characteristics limit clinical efficacy.Thus,the establishment of better biomarkers is expected.Fucosylation is one of the most important glycosylation modifications of proteins.Fucosylated haptoglobin(Fuc-Hpt)is used as a biomarker for several cancers and inflammation-related diseases.We recently established a novel glycan monoclonal antibody(mAb),designated 10-7G,which recognizes Fuc-Hpt.We developed an enzyme-linked immunosorbent assay(ELISA)to measure serum levels of Fuc-Hpt(10-7G values).AIM To investigate the usefulness of the serum 10-7G values as a potential biomarker for monitoring disease activity in IBD.METHODS This was a case control study.Intestinal tissues of IBD patients(n=10)were examined immunohistochemically using the 10-7G mAb.We determined 10-7G values using serum from patients with ulcerative colitis(UC,n=110),Crohn’s disease(n=45),acute enteritis(AE,n=11),and healthy volunteers(HVs)who exhibited normal(n=20)or high(n=79)C-reactive protein(CRP)levels at medical check-up.We investigated the correlation between the 10-7G value and various clinical parameters of IBD patients by correlation analysis.Receiver operating characteristic(ROC)curve analysis was performed to evaluate the usefulness of the 10-7G values as a biomarker for clinical and endoscopic remission of UC compared to conventional serum biomarkers.RESULTS In the immunohistochemical analysis,positive 10-7G mAb staining was observed in lymphocytes infiltrating into inflammatory sites of the mucosal layer and lymphoid follicles.The 10-7G values were significantly higher in patients with IBD(P<0.001)and AE(P<0.05)compared with HVs.In addition,10-7G values were correlated with clinical examination parameters related to inflammation in patients with UC,particularly the CRP level(rs=0.525,P=0.003

关 键 词：Inflammatory bowel disease Biomarkers GLYCOSYLATION Fucosylated haptoglobin Prohaptoglobin Endoscopic remission 

分 类 号：R574[医药卫生—消化系统]