不同氮/碳源组合条件下斜生栅藻的生长、光合及油脂产率  

Growth,photosynthesis and total lipid of Scenedesmus obliquus under different nitrogen and carbon source combinations

作　　者：杨宋琪 魏喜红 王丽娟[1] 杨生辉[1] 罗光宏[1] YANG Songqi;WEI Xihong;WANG Lijuan;YANG Shenghui;LUO Guanghong(Key Laboratory of Hexi Corridor Resources Utilization of Gansu,Gansu Engineering Technology Innovation Center for Microalgae,Hexi University,Zhangye 734000,Gansu,China;Key Laboratory of Eco-environments in Three Gorges Reservoir Region,Southwest University,Chongqing 400715,China)

机构地区：[1]河西学院甘肃省微藻工程技术创新中心,甘肃省河西走廊特色资源利用重点实验室,甘肃张掖734000 [2]西南大学三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《中国油脂》2020年第12期112-117,共6页China Oils and Fats

基　　金：甘肃省科技计划资助(18JR2JG001,18YF1WG087,20JR5RE646)。

摘　　要：以斜生栅藻为研究材料,选取NaNO3、NaNO2、NH4Cl、尿素(CO(NH2)2)作为氮源,Na2CO3、C6H12O和CH3COONa作为碳源,进行两两组合培养,研究不同氮源、碳源组合条件对斜生栅藻生长、最大光量子产率(Fv/Fm)、油脂产率及脂肪酸组成的影响。结果表明:CO(NH2)2作为氮源时,斜生栅藻生物量、叶绿素a含量及Fv/Fm均达到最高,而NH4Cl不利于斜生栅藻的生长。在NaNO3、NaNO2和NH4Cl为氮源时,3种碳源对斜生栅藻生长的影响差异不显著(P>0.05),而在CO(NH2)2环境中,Na2CO3的添加使得斜生栅藻生物量显著高于其他两组(P<0.05),说明CO(NH2)2和Na2CO3组合更有利于斜生栅藻的生长。12组处理中,N1(CO(NH2)2+Na2CO3)和Y2(NaNO2+C6H12O)油脂产率最高,分别占细胞干重的23.14%和21.54%,同时藻油中油酸相对含量分别为57.92%和57.81%。因此,无论是生物量的积累还是油脂产率,CO(NH2)2作为氮源且Na2CO3作为碳源具有一定的应用前景。Using Scenedesmus obliquus as the research material,NaNO3,NaNO2,NH4Cl,urea(CO(NH2)2)as nitrogen sources and Na2CO3,C6H12O and CH3COONa as carbon sources,the effects of different nitrogen and carbon sources on the growth,Fv/Fm,total lipid and fatty acid composition of S.obliquus were studied.The results showed that the biomass,chlorophyll a content and Fv/Fm of S.obliquus reached the highest value when the CO(NH2)2 was used as the nitrogen source,while the nitrogen source of NH4Cl was not beneficial to the growth of S.obliquus.When NaNO3,NaNO2 and NH4Cl were used as the nitrogen sources,the effects of three carbon sources on the growth of S.obliquus were not significant(P>0.05).In the CO(NH2)2 environment,the addition of Na2CO3 significantly increased the biomass of S.obliquus compared to the other two groups(P<0.05).It indicated that the combination of CO(NH2)2 and Na2CO3 was more conductive to the growth of S.obliquus.Among all the twelve treatments,N1(CO(NH2)2+Na2CO3)and Y2(NaNO2+C6H12 O)had the highest total lipid,accounting for 23.14%and 21.54%of the dry cell weight,respectively,while the relative contents of oleic acid in algae oils were 57.92%and 57.81%respectively.Therefore,using CO(NH2)2 as the nitrogen source and Na2CO3 as the carbon source had certain application prospects for either the biomass accumulation or the lipid production of S.obliquus.

关 键 词：斜生栅藻 氮源 碳源 生长 油脂 

分 类 号：TQ642[化学工程—精细化工] S985.4[农业科学—捕捞与储运]