异氟醚对脑缺血再灌注大鼠侧支循环形成及Notch通路的影响  

Effects of Isoflurane on the Formation of Collateral Circulation and Notch Pathway in Rats with Cerebral Ischemia-reperfusion

作　　者：张敏[1] 项明琼 油文静 伍璀 ZHANG Min;XIANG Mingqiong;YOU Wenjing(Yangpu District Central Hospital Affiliated to Tongji University, Shanghai 200082, China)

机构地区：[1]同济大学附属杨浦区中心医院麻醉科,上海200082 [2]复旦大学附属华山医院北院麻醉科,上海200082

出　　处：《河北医学》2021年第1期5-11,共7页Hebei Medicine

基　　金：上海市科学技术委员会科研计划项目,(编号:18411932500)。

摘　　要：目的:探讨异氟醚对脑缺血再灌注大鼠侧支循环形成及Notch通路的影响。方法:清洁级SD大鼠90只随机分成:对照组、模型组、依达拉奉组(3mg/kg)、异氟醚低剂量组(50μg/kg)、异氟醚高剂量组(100μg/kg),每组18只;模型组、依达拉奉组、异氟醚低、高剂量组建立大鼠脑缺血再灌注模型,依达拉奉组、异氟醚低、高剂量组建模成功后第1天开始腹腔注射相应药物,每天1次,持续给予1周,对照组和模型组腹腔注射等体积生理盐水。实验结束后,测定大鼠脑缺血再灌注区域微血管密度,神经运动功能评分,获取双侧贴纸去除及平衡木过杆实验结果,观察大鼠脑缺血再灌注区域神经元结构,实时荧光定量逆转录-多聚酶链反应(RT-PCR)法及蛋白质免疫印迹(Western-blot)法测定Notch1、血管内皮生长因子(VEGF)mRNA和蛋白水平。结果:对照组神经元细胞完整;模型组海马区可见大量坏死神经元,神经细胞核固缩,有明显炎症细胞浸润;依达拉奉组、异氟醚高剂量组神经元细胞结构较为完整,炎症细胞浸润减少;异氟醚低剂量组仍可见明显炎症细胞浸润。与对照组比较,模型组脑缺血再灌注区域微血管密度、Notch1、VEGF mRNA和蛋白水平降低,神经运动功能评分、双侧贴纸去除时间、平衡木过杆时间升高(P<0.05)。与模型组比较,依达拉奉组、异氟醚低剂量组、异氟醚高剂量组脑缺血再灌注区域微血管密度、Notch1、VEGF mRNA和蛋白水平升高,神经运动功能评分、双侧贴纸去除时间、平衡木过杆时间降低(P<0.05);且异氟醚高剂量组脑缺血再灌注区域微血管密度、Notch1、VEGF mRNA和蛋白水平高于异氟醚低剂量组,神经运动功能评分、双侧贴纸去除时间、平衡木过杆时间低于异氟醚低剂量组(P<0.05)。异氟醚高剂量组脑缺血再灌注区域微血管密度、Notch1、VEGF mRNA和蛋白水平、神经运动功能评分、双侧贴纸去除时间、平�Objective:To investigate the effects of isoflurane on the formation of collateral circulation and Notch pathway in rats with cerebral ischemia-reperfusion.Methods:90 clean grade SD rats were random divided into control group,model group,edaravone group(3 mg/kg),isoflurane low-dose group(50μg/kg),isoflurane high-dose group(100μg/kg),18 in each group.The rats in the model group,edaravone group,isoflurane low-dose group and high-dose group were established cerebral ischemia-reperfusion model.After successful modeling,the rats in the edaravone group,isoflurane low-dose group and high-dose group on the first day after operation were intraperitoneally administrated corresponding drugs,once a day,for 1 week.The rats in the control group and the model group were given the same volume of saline.After the experiment,the microvessel density and neuromotor function scores in the reimplantation area of the rat brain were measured,and the results of the bilateral sticker removal and balance beam crossing experiment were obtained.The neuron structure in the reimplantation area of the rat brain injury was observed.And real-time fluorescence quantitative reversal Record-polymerase chain reaction(RT-PCR)and Western-blot(Western-blot)method to measure Notch1,vascular metabolic growth factor(VEGF)mRNA and protein levels.Results:The neuron cells in the control group were intact;a large number of necrotic neurons were seen in the hippocampus of the model group,the nerve cell nucleus was contracted,and there was significant inflammatory cell infiltration;the neuronal cell structure in the edaravone group and the isoflurane high-dose group was relatively complete,and the inflammatory cell infiltration Reduced;significant infiltration of inflammatory cells is still visible in the low-dose isoflurane group.Compared with the control group,the microvascular density in cerebral ischemia-reperfusion area,Notch1,VEGF mRNA and protein levels in the model group were decreased,and the neuromotor function score,the removal time of bilateral stic

关 键 词：异氟醚 脑缺血再灌注 侧支循环 NOTCH1 血管内皮生长因子 

分 类 号：R73[医药卫生—肿瘤]