碱性电解水提取灵芝子实体多糖的工艺优化  

Optimization of Alkaline Electrolyzed Water Extraction Process of Ganoderma lucidum Polysaccharides by Response Surface Methodology

作　　者：苗雨欣 刘旭 宁慧娟[1] 葛鑫会 张京声[1] 孙君社[1] 张秀清[1] MIAO Yu-xin;LIU Xu;NING Hui-juan;GE Xin-hui;ZHANG Jing-sheng;SUN Jun-she;ZHANG Xiu-qing(College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 10000,China)

机构地区：[1]中国农业大学食品科学与营养工程学院,北京100083

出　　处：《中国食用菌》2020年第11期62-67,74,共7页Edible Fungi of China

基　　金：2018年国家自然科学基金项目(21576142)。

摘　　要：采用Box-Behnken设计的响应曲面法对灵芝多糖的碱性电解水提取工艺进行优化。在单因素试验的基础上,选取料液比、电解水pH和沸水浴提取时间3个因素进行响应曲面分析,得到灵芝子实体多糖的碱性电解水提取工艺优化条件为:料液比1∶60 (g∶mL),电解水pH 12.5,沸水浴提取时间105 min,此条件下测得灵芝多糖得率为(3.11±0.10)%。该工艺提取得到的多糖在AFM显微镜下呈现长度为314 nm^372 nm、高为2.80nm^4.30 nm的棒状纳米颗粒。The response surface method designed by Box-Behnken was used to optimize the alkaline electrolytic water extraction process of Ganoderma lucidum polysaccharides. Based on the single-factor test, the optimized conditions for the alkaline electrolytic water extraction of Ganoderma lucidum fruiting body polysaccharides were as following: the material-liquid ratio was1 ∶ 60(g ∶ mL), the pH of the electrolyzed water was 12.5, and the extraction time in a boiling water bath was 105 minutes. Under these conditions, the polysaccharide yield was(3.11 ± 0.10)%. Under the AFM microscope, the polysaccharide extracted by this process showed rod-shaped nanoparticles with a length of 314 nm to 372 nm and a height of 2.80 nm to 4.30 nm.

关 键 词：灵芝多糖 碱性电解水 响应面法 

分 类 号：S646.9[农业科学—蔬菜学]