一株伯克霍尔德菌所产类细菌素的分离纯化  

Isolation and purification of bacteriocin-like produced by Burkholderia

作　　者：刘静 杨富民[1] 王丽君 苏阿伦 LIU Jing;YANG Fumin;WANG Lijun;SU Alun(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China;Gansu Province Food Inspection Institute,Lanzhou 730030,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070 [2]甘肃省食品检验研究院,甘肃兰州730030

出　　处：《中国酿造》2021年第1期138-141,共4页China Brewing

基　　金：甘肃省科技计划资助(20JR5RA168)。

摘　　要：利用硫酸铵分级沉淀、固相萃取小柱层析、高效液相色谱分离的方法纯化一株伯克霍尔德菌所产类细菌素。结果表明,纯化效果较好,在保留时间5.783 min时出现一个较强的单一吸收峰,抑菌物质的最大吸收波长为223.06 nm,蛋白质含量为452μg/mL。The bacteriocin-like produced by Burkholderia were purified by ammonium sulfate precipitation,solid phase extraction and high performance liquid chromatography(HPLC)isolation.The results showed that the purification effect was good,and a strong single absorption peak appeared at the retention time of 5.783 min.The maximum absorption wavelength of antibacterial substances was 223.06 nm,and the protein content was 452μg/ml.

关 键 词：固相萃取小柱 高效液相色谱法 纯化 伯克霍尔德菌 

分 类 号：TS262.3[轻工技术与工程—发酵工程]