菊苣多糖对免疫抑制小鼠免疫功能的影响  

Effect of Chicory Polysaccharide on Immune Function in Immunosuppressed Mice

作　　者：吴雨龙 朱华 张艺鏻 江海涛 华春 WU Yulong;ZHU Hua;ZHANG Yilin;JIANG Haitao;HUA Chun(School of Food Science,Nanjing Xiaozhuang University,Nanjing 211171,China;Jiangsu Provincial Key Construction Laboratory of Special Biomass Waste Resource Utilization,Nanjing 211171,China;Jiangsu Second Normal College,Nanjing 210013,China)

机构地区：[1]南京晓庄学院食品科学学院,江苏南京211171 [2]江苏省高校“特殊生物质废弃物资源化利用”重点建设实验室,江苏南京211171 [3]江苏第二师范学院,江苏南京210013

出　　处：《食品工业科技》2021年第3期284-289,337,共7页Science and Technology of Food Industry

基　　金：国家高技术研究发展计划(2012AA021701);国家自然科学基金(21376112);江苏省自然科学基金(BK20141081);江苏省高校自然科学研究重大项目(18KJA180007);江苏省高等学校大学生创新创业训练计划项目(201911460020Z);南京晓庄学院青年专项(2019NXY47)。

摘　　要：目的:研究菊苣多糖(chicory polysaccharide,CP)对免疫抑制小鼠免疫功能的影响。方法:ICR小鼠随机分为空白组、模型组、药物组(脾氨肽)、CP低、中、高剂量组。除空白组外,其余各组用环磷酰胺造成免疫抑制小鼠模型。分别测定各组小鼠体质量、免疫器官指数、外周血白细胞和红细胞数量、迟发型超敏反应致小鼠足趾肿胀度、腹腔巨噬细胞吞噬能力、血清血溶素水平及血清中白细胞介素2(IL-2)、白细胞介素6(IL-6)以及肿瘤坏死因子α(TNF-α)的活性水平。结果:与模型组相比,CP高剂量组能显著提高免疫抑制小鼠的净体质量增率、免疫器官指数、巨噬细胞吞噬率和吞噬指数、白细胞和红细胞数量以及降低足趾肿胀度(P<0.05)。同时,CP高剂量组还能明显提高小鼠腹腔巨噬细胞吞噬能力、血清溶血素含量,调节免疫抑制小鼠的免疫细胞因子。结论:CP对免疫抑制小鼠具有增强免疫活性的作用。Objective:To study the effect of chicory polysaccharide on immune function in immunosuppressed mice.Methods:ICR mice were randomly divided into blank group,model group,drug group(Piantai),CP low,medium and high dose group.In addition to the blank group,the other groups used cyclophosphamide to cause immunosuppressed mouse model.Body weight,immune organ index,peripheral blood leukocytes and erythrocyte count,delayed hypersensitivity-induced toe swelling,peritoneal macrophage phagocytosis,serum levels of lysin and activity levels of interleukin 2(IL-2),interleukin 6(IL-6)and tumor necrosis factorα(TNF-α)in each group of mice were determined,respectively.Results:Compared with the model group,CP high dose group could significantly increase net body weight rate,immune organ index,macrophage phagocytosis rate and index,number of leukocytes and erythrocyte,and decrease toe swelling in immunosuppressed mice(P<0.05).Meanwhile,CP high dose group could also significantly improve phagocytosis of mouse peritoneal macrophages,serum lysin content,and regulate immune cytokines in immunosuppressed mice.Conclusion:CP could enhance immune activity in immunosuppressed mice.

关 键 词：菊苣多糖 免疫抑制 免疫活性 调节 

分 类 号：TS201.1[轻工技术与工程—食品科学]