植物乳杆菌对苜蓿青贮发酵品质和细菌菌群的影响  

Effect of Lactobacillus plantarum on Fermentation Quality and Bacterial Flora in Alfalfa Silage

作　　者：王媛[1,2] 王雁萍 赵珊珊[1,2] 杨逢源[1,2] 郝湘妹 范小苗 WANG Yuan;WANG Yanping;ZHAO Shanshan;YANG Fengyuan;HAO Xiangmei;FAN Xiaomiao(School of Physics,Zhengzhou University,Zhengzhou 450001, China;Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou 450001, China;School of Agricultural Sciences, Zhengzhou University,Zhengzhou 450001, China)

机构地区：[1]郑州大学物理学院,河南郑州450001 [2]河南省离子束生物工程重点实验室,河南郑州450001 [3]郑州大学农学院,河南郑州450001

出　　处：《郑州大学学报（理学版）》2021年第1期114-119,共6页Journal of Zhengzhou University:Natural Science Edition

基　　金：国家自然科学基金项目(31772672);郑州大学横向项目(20190280A)。

摘　　要：为了探究植物乳杆菌(Lactobacillus plantarum)作为添加剂对苜蓿青贮发酵品质与细菌菌群的影响,以新鲜苜蓿为原料,分别添加植物乳杆菌YX(LPI)或ZZU A341(LPA),以无添加剂为对照组。青贮60 d后取样分析发酵品质,并利用高通量测序技术分析细菌菌群变化情况。结果表明:青贮60 d后,与对照组相比,LPI或LPA组的细菌菌群多样性、pH值和干物质损失率均显著降低(P<0.05),干物质含量和乳酸含量显著升高(P<0.05);LPI或LPA组中Garciella属和Weissella属的相对丰度较低,Lactobacillus属的相对丰度较高,且细菌菌群组成发生改变;环境因子分析显示,pH值和氨态氮含量与Garciella属和Weissella属呈正相关,与Lactobacillus属呈负相关。In order to explore the effect of Lactobacillus plantarum inoculant on fermentation quality and bacterial flora in alfalfa silage,fresh alfalfa was was used as raw material,Lactobacillus plantarum YX(LPI)or Lactobacillus plantarum ZZU A341(LPA)were added respectively,and no addition was used as control(CK).After 60 d,fermentation quality and microbial species in silages were analyzed by high-throughput sequencing.Compared to CK group,bacterial diversity,pH value and dry matter loss of LPI or LPA group were significantly decreased(P<0.05),while dry matter content and lactic acid content were significantly increased(P<0.05).The inoculated silages also exhibited lower abundance of Garciella and Weissella,and higher abundance of Lactobacillus.Besides,the distribution of the bacterial community was shifted.Environmental factor analysis showed that pH and AN content had positive correlations with Garciella and Weissella,and negative correlations with Lactobacillus.

关 键 词：苜蓿青贮 细菌菌群 高通量测序 植物乳杆菌 

分 类 号：Q939.99[生物学—微生物学]