出芽短梗霉产黑色素发酵工艺优化  

Optimization of Fermentation Technology of Melanin from Aureobasidium pullulans

作　　者：魏永昌 袁凯凯 李振海 仝雅娜 乔长晟[1,2,3] WEI Yong-chang;YUAN Kai-kai;LI Zhen-hai;TONG Ya-na;QIAO Chang-sheng(College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457,China;Tianjin Key Laboratory of Industrial Microbiology,Education Ministry Key Laboratory of Industrial Fermentation Microbiology,Tianjin 300457,China;Tianjin Peiyang Biotrans Biotechnology Company,Tianjin 300457,China;Tianjin Facility Agriculture Research Institute,Tianjin 300112,China)

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]天津市工业微生物重点实验室,工业发酵微生物教育部重点实验室,天津300457 [3]天津北洋百川生物技术有限公司,天津300457 [4]天津市设施农业研究所,天津300112

出　　处：《食品研究与开发》2021年第3期170-176,共7页Food Research and Development

基　　金：天津市科技计划项目(17YFCZZC00310)。

摘　　要：该文以出芽短梗霉(Aureobasidium pullulans)为生产菌株,通过单因素和响应面试验对培养基组分进行优化以提高黑色素产量。结果表明,出芽短梗霉发酵产黑色素的最佳培养基组成为:蔗糖98.9 g/L、玉米浆干粉9.7 g/L、CaCl2·2H2O 2.0 g/L、NaCl 2.0 g/L、ZnSO41.0 g/L、pH 5.5。在此条件下,经过144 h发酵,黑色素产量达到17.56 g/L,相比优化之前提高了42.8%。Based on a strain of Aureobasidium pullulans,an optimization on medium components was performed by means of single-factor experiment and response surface method to improve melanin yield.The results showed that the optimum medium components were as below that sucrose 98.9 g/L,corn steep flour 9.7 g/L,CaCl2·2H2O 2.0 g/L,NaCl 2.0 g/L,ZnSO41.0 g/L,pH 5.5.Under above described conditions,a desirable melanin yield of 17.56 g/L was achieved after 144 h fermentation which was 42.8%up than that before optimization.

关 键 词：出芽短梗霉 黑色素 发酵工艺 培养基优化 响应面 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]