Neurospora crassa产木聚糖酶发酵条件及酶学特性  

Optimization of Fermentation Conditions and Enzymatic Properties of Xylanase from Neurospora Crassa

作　　者：曹慧[1] 张朋振 张腾月 程鹏 杨佳萌 任世威 井佳骐 CAO Hui;ZHANG Pengzhen;ZHANG Tengyue;CHENG Peng;YANG Jiameng;REN Shiwei;JING Jiaqi(College of Animal Science and Technology,Henan University of Science and Technology,Henan Luoyang 471000,China;College of Agriculture,Guangdong Ocean University,Guangdong Zhanjiang 524088,China)

机构地区：[1]河南科技大学动物科技学院,河南洛阳471000 [2]广东海洋大学农学院,广东湛江524088

出　　处：《饲料工业》2021年第2期34-40,共7页Feed Industry

基　　金：河南科技大学博士科研启动资金[13480077];青海省重点研发计划[2017-NK-153、2019-SF-149、2019-NK-173]。

摘　　要：从青藏高原土壤中筛选出一株产木聚糖酶较高的菌株Neurospora crassa SD10。通过单因素试验和正交试验对菌株进行发酵条件优化和酶学性质探究。结果表明,菌株SD10最佳发酵条件为:麸皮30 g/L、蛋白胨20 g/L、Mn^2+0.5 g/L、培养温度33℃、转速230 r/min、接种量2%、初始pH值8.0、发酵时间84 h。菌株产木聚糖酶的最适反应温度为50℃,最适反应pH值为6.0,在40~50℃和pH值4.0~8.0时有较好的稳定性,能保持80%以上酶活力,Fe^2+、Zn^2+、K+对酶活力有促进作用,Na^+、Mg^2+、Ca^2+、Mn2^+、Cu^2+对酶活力有抑制作用。Neurospora crassa SD10优化后产木聚糖酶活力达9.4 U/mL,所产木聚糖酶具有良好的pH值稳定性。Neurospora crassa SD10 was the strain with high xylanase activity from the alpine mead⁃ow soil on the Tibetan Plateau.The fermentation condition and enzymatic properties were conduct⁃ed by single factor and orthogonal test.The re⁃sults showed that the optimum fermentation conditions were as follows:bran 30 g/L,peptone 20 g/L,Mn2+0.5 g/L,33℃,pH 8.0,inoculum amount 2%,rotation speed 230 r/min after 84 h.The enzyme was optimally active at temperature 50℃and pH 6.0.The enzyme activity was relative stable under 40~50℃or pH 4.0~8.0.The xylanase activity of SD10 was enhanced by Fe2+,Zn2+,K+,but reduced by Na+,Mg2+,Ca2+,Mn2+,Cu2+.The highest xylanase activity of Neurospora crassa SD10 reached 9.4 U/mL by optimization.And the xylanase have good pH stability.

关 键 词：木聚糖酶 产酶条件优化 酶学性质 Neurospora crassa 青藏高原 

分 类 号：S816.32[农业科学—饲料科学]