Conversion of D-glucose to L-lactate via pyruvate by an optimized cell-free enzymatic biosystem containing minimized reactions  

作　　者：Leipeng Xie Xinlei Wei Xigui Zhou Dongdong Meng Ruimin Zhou Yi-Heng P.Job Zhang Shuxia Xu Chun You 

机构地区：[1]College of Life Sciences,Henan Agricultural University,95 Wenhua Road,Zhengzhou,450002,China [2]Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,32 West 7th Avenue,Tianjin Airport Economic Area,Tianjin,300308,China

出　　处：《Synthetic and Systems Biotechnology》2018年第3期204-210,共7页合成和系统生物技术（英文）

基　　金：the Key Research Program of the Chinese Academy of Sciences(Grant No.ZDRW-ZS-2016-3);National Natural Science Foundation of China(Grant No.31600636).

摘　　要：Cell-free synthetic enzymatic biosystem is emerging to expand the traditional biotechnological mode by utilizing a number of purified/partially purified enzymes and coenzymes in a single reaction vessel for the production of desired products from low-cost substrates.Here,a cell-free synthetic biosystem containing minimized number of reactions was designed for the conversion of D-glucose to L-lactate via pyruvate.This NADH-balanced biosystem was comprised of only 5 thermophilic enzymes without ATP supplementation.After optimization of enzyme loading amounts,buffer concentration and cofactor concentration,D-glucose was converted to L-lactate with a product yield of∼90%.Our study has provided an emerging platform with potentials in producing pyruvatederived chemicals,and may promote the development of cell-free synthetic enzymatic biosystems for biomanufacturing.

关 键 词：Cell-free synthetic enzymatic biosystem Minimized reactions Thermophilic enzymes L-LACTATE PYRUVATE Biomanufacturing 

分 类 号：Q95[生物学—动物学]