Shiqi herbal tea reduces the susceptibility to H1N1 influenza virus in stressed mice  

作　　者：Guo Xie Zhuo Luo Lu-Ping Tang Li-Hang Zhang Li-Hua Peng Ling Jin Hiroshi Kurihara Jin-Le Cheng Yi-Fang Li Rong-Rong He 

机构地区：[1]Zhongshan Institute,University of Electronic Science and Technology of China,Zhongshan 528402,Guangdong,China [2]International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Chinese Ministry of Education(MOE),College of Pharmacy,Jinan University,Guangdong,China [3]Key Laboratory of Technology and Application of Ultrafine Granulated Powder of Traditional Chinese Medicine,State Administration of Traditional Chinese Medicine of the People's Republic of China,Zhongshan 528437,Guangdong,China [4]Guangdong Province Research and Development Center for Chinese Medicine in Disease Susceptibility,College of Pharmacy,Jinan University,Guangdong,China [5]Department of Traditional Chinese Medicine,The First Affiliated Hospital,Jinan University,Guangdong,China

出　　处：《TMR Modern Herbal Medicine》2021年第1期44-52,共9页TMR现代中药

基　　金：This work was financially supported,in part,by Science and Technology Project of Zhongshan(2013A3FC0253);Scientific Research Team Training Project in University of Electronic Science and Technology of China,Zhongshan Institute(No.412YT02);Natural Science Foundation of Guangdong Province-the Ph.D.Startup and Vertical Collaboration Project(2018030310186).

摘　　要：Objective:It is well known that stress plays a critical role in immune response and susceptibility to diseases.Among many stressors,restraint stress has been shown to suppress immune function and increase susceptibility to infections.In this study,we employed a restraint-mouse model to investigate the effect and preliminary mechanism of ShiQi herbal tea(SQHT),a traditional Chinese medicine,on H1N1 virus infection.Methods:Mice were exposed to restraint stress and infected with H1N1 influenza virus by intranasal inoculation.SQHT(936 and 1872 mg/kg/d)was orally administrated to mice for 7 days from the first day of restraint stress.The survival rate of mice in each group was monitored daily for 21 days.Histopathological changes,inflammatory cells infiltration,and virus titer in lungs were examined.For the study of mechanisms,we investigated whether SQHT could promote interferon-β(IFN-β)generation and interferon stimulated genes(ISG)expression.Results:Our results suggested that SQHT(936 mg/kg/d)significantly reduced H1N1-induced mortality,the level of complement C5a and lung tissue inflammation,and viral replication in restraint-stressed mice.Further results revealed that in restraint-stressed mice model,SQHT(936 mg/kg/d)administration markedly improved IFN-βgeneration,and increased MX1 and IFITM3 gene expression.Conclusion:Our study demonstrates that SQHT reduces restraint stress-induced susceptibility to H1N1 infection via improving IFN-βantiviral pathway,which provides a certain basis for the clinical use of SQHT to treat H1N1 infection.

关 键 词：ShiQi herbal tea Restraint stress H1N1 virus C5A INTERFERON-Β SUSCEPTIBILITY 

分 类 号：R51[医药卫生—内科学]