Enhanced osteogenesis and therapy of osteoporosis using simvastatin loaded hybrid system  

作　　者：Tao Wu Jing Sun Lei Tan Qi Yan Lei Li Liangwen Chen Xiangmei Liu Shi Bin 

机构地区：[1]The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine Ministry of Education,Department of Dental Implantology,School&Hospital of Stomatology,Wuhan University,237 Luoyu Road,Wuhan,430079,PR China [2]Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials,Hubei Key Laboratory of Polymer Materials,School of Materials Science&Engineering,Hubei University,Wuhan,430062,China

出　　处：《Bioactive Materials》2020年第2期348-357,共10页生物活性材料（英文）

基　　金：supported by National Natural Science Foundation of China(Grant 81600906);the Fundamental Research Funds for the Central University of China(No.2042018kf0108).

摘　　要：Postmenopausal osteoporosis is a common chronic dynamic bone disorder,caused by estrogen deficiency.To address this issue,we constructed a controlled drug-release system composed of poly(N-isopropylacrylamide)brush modified mesoporous hydroxyapatite(MHA-SIM-P)loaded with simvastatin(SIM)using an ovariectomised(OVX)rat model.Quantitative alkaline phosphatase activity assay,alizarin red staining and RT-PCR were tested to evaluate the osteogenic ability in vitro.The results showed that the MHA-SIM-P nanoparticles significantly improved the osteogenic differentiation of OVX bone marrow stromal cells(BMSCs)in vitro.In osteoporotic animal model,the therapeutic efficiency for bone defect was evaluated byμCT analysis,tartrateresistant acid phosphatase,haematoxylin and eosin staining,which showed improved bone formation and less osteoclastic response in OVX rats after surgery for 3 and 6 weeks.This polymer brush modified MHA system provided a sustained release system of hydrophobic SIM to inhibit osteoporosis together with MHA nanoparticle promoting the osteogenesis.Thus,this novel strategy exhibited great potential for promoting osteogenic ability and treating local osteoporotic defects.

关 键 词：OSTEOGENESIS OSTEOPOROSIS Mesoporous hydroxyapatite Bone marrow stromal cells Drug release 

分 类 号：R68[医药卫生—骨科学]