Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application  

作　　者：Peng Gao Bo Fan Xiaoming Yu Wenwen Liu Jie Wu Lei Shi Di Yang Lili Tan Peng Wan Yulin Hao Shujun Li Wentao Hou Ke Yang Xiaokang Li Zheng Guo 

机构地区：[1]Department of Orthopedics,Xijing Hospital,Fourth Military Medical University,Xi'an,710032,China [2]Department of Joint Surgery and Sports Medicine,Hunan Provincial People's Hospital and The First Affiliated Hospital of Hunan Normal University,Changsha,410016,PR China [3]Institute of Metal Research,Chinese Academy of Science,Shenyang,110016,China [4]School of Material Science and Engineering,Shenyang Ligong University,Shenyang,110159,China

出　　处：《Bioactive Materials》2020年第3期680-693,共14页生物活性材料（英文）

基　　金：financially supported by The National Key Research and Development Program of China(grant number 2017YFC1104901);The National Natural Science Foundation of China(grant number 51871239,51771227);National Natural Science Foundation of Youth Fund(grant number 51501223).

摘　　要：The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application.Early angiogenesis is important for scaffold survival.It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties.In this study,a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold.For osseointegration and osteogenesis analysis,in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation,adhesion,extracellular matrix(ECM)mineralization and ALP activity compared with bare Ti6Al4V cocultivation.Additionally,MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesisrelated genes expression.In vivo studies including fluorochrome labeling,micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation.For angiogenesis studies,magnesium-coated Ti6Al4V improve HUVECs proliferation,adhesion,tube formation,wound-healing and Transwell abilities.HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes(HIF-1αand VEGF)expression.Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation.This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications.

关 键 词：Porous Ti6Al4V scafflod Surface modification Magnesium coating OSTEOGENESIS ANGIOGENESIS 

分 类 号：R318[医药卫生—生物医学工程] R68[医药卫生—基础医学]