Calcium-mineralized polypeptide nanoparticle for intracellular drug delivery in osteosarcoma chemotherapy  

作　　者：Ke Li Di Li Li Zhao Yonghe Chang Yi Zhang Yan Cui Zhiyu Zhang 

机构地区：[1]Department of Orthopedics,The Fourth Affiliated Hospital of China Medical University,4 Chongshandong Road,Shenyang 110032,People's Republic of China [2]Key Laboratory of Polymer Ecomaterials,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,5625 Renmin Street,Changchun 130022,People's Republic of China [3]Laboratory of Building Energy-Saving Technology Engineering,College of Material Science and Engineering,Jilin Jianzhu University,5088 Xincheng Street,Changchun 130118,People's Republic of China

出　　处：《Bioactive Materials》2020年第3期721-731,共11页生物活性材料（英文）

基　　金：financially supported by the National Natural Science Foundation of China(Grant No.51803006);the Scientific Development Program of Liaoning Province(Grant No.20170541058);the China Postdoctoral Science Foundation(Grant No.2019M650297).

摘　　要：The acidic microenvironments of tumor tissue and cells provide an opportunity for the development of pHresponsive drug delivery systems in cancer therapy.In this work,we designed a calcium carbonate(CaCO3)-corecrosslinked nanoparticle of methoxy poly(ethylene glycol)-block-poly(L-glutamic acid)through mineralization for intracellular delivery of doxorubicin(DOX),referred to as CaNP/DOX.CaNP/DOX exhibited high drug loading capability,uniform nanoparticle size,and pH-dependent DOX release.In the meantime,the enhanced cell uptake,superior cytotoxicity toward mouse osteosarcoma K7 cells,extended circulation half-life,and improved accumulation of DOX in K7 allograft tumor from CaNP/DOX were also demonstrated.More interestingly,CaNP/DOX displayed improved antitumor effect and reduced side effects against the K7 osteosarcoma-allografted mouse model and the 143B orthotopic osteosarcoma mouse model.Given the superior properties of Ca-mineralized polypeptide nanoparticle for intracellular drug delivery,the smart drug delivery system showed strong competitiveness in clinical chemotherapy of cancers.

关 键 词：POLYPEPTIDE Calcium mineralization Controlled drug release pH-responsiveness Osteosarcoma chemotherapy 

分 类 号：R730.5[医药卫生—肿瘤] TB383[医药卫生—临床医学]