High uric acid model in Caenorhabditis elegans  

作　　者：Zhenjing Li Yibin Xue Nifei Wang Jingli Cheng Xiaoying Dong Qingbin Guo Changlu Wang 

机构地区：[1]State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457,China [2]Key Laboratory of Food Nutrition and Safety,Ministry of Education,School of Food Engineering and Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China [3]Department of nephrology,Metabolic Disease Hospital,Tianjin Medical University,Key Laboratory of Hormones and Development,Ministry of Health,Tianjin 300070,China

出　　处：《Food Science and Human Wellness》2019年第1期63-66,共4页食品科学与人类健康（英文）

基　　金：a grant from the Scientific Research Fund of Tianjin University of Science and Technology(No.20120105);National Natural Science Foundation of China(No.31701551).

摘　　要：To establish experimental high uric acid model in C.elegans.Hypoxanthine,adenine,xanthine,and uric acid were used to treat C.elegans and then hyperuricemic C.elegans was evaluated by allopurinol.Hyperuricemic C.elegans were obtained after normal worms were treated by xanthine(0.25 mg/mL,18 h).For hyperuricemic worms,there was a statistically significant increase in the uric acid level(p<0.001)and a lower drug damage(p>0.05).Moreover,the model was proved to keep a high uric acid level for up to 12 h.After given allopurinol(0.25 mg/mL,12 h),the uric acid of hyperuricemic C.elegans had a significant reduction by 15%.Furthermore,xanthine oxidase activity in hyperuricemic C.elegans showed a statistically significant increase(p<0.001),which resulted in a raised uric acid content.A high uric acid model with low drug damage and high efficiency and stability was established in C.elegans after simply xanthine treatment.

关 键 词：HYPERURICEMIA C.elegans Uric acid XANTHINE Xanthine oxidase 

分 类 号：TQ460.1[化学工程—制药化工]