Study on the Photodynamic Efficiency of Sodium Chlorophyllin from Spirulina  

作　　者：Jianjiao CHEN Zhiqiang CHEN Jun WEN Jian WEN Jingfei FENG Jing YI Dengfeng ZOU 

机构地区：[1]Guilin Medical University,Guilin 541000,China [2]Affiliated Hospital of Guilin Medical University,Guilin 541000,China

出　　处：《Agricultural Biotechnology》2021年第1期133-135,共3页农业生物技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(32060228);Guangxi Natural Science Foundation(2017GXNSFAA198112;2019GXNSFAA245077);Guangxi Graduate Education Innovation Project(GJY2018116;YCSW2019214;YCSW2020225);Undergraduate Innovation Training Program(202010601164;202010601093)。

摘　　要：[Objectives] This study was conducted to investigate the photodynamic technology(PDT) of water-soluble sodium chlorophyllin extract from Spirulina and its photodynamic sterilization efficiency on Gram bacteria and photodynamic antitumor effect on rat glioma C6 cells. [Methods]The absorption spectrum and fluorescence spectrum of sodium chlorophyllin were measured by an ultraviolet spectrophotometer and a fluorescence spectrophotometer;the plate count method was used to investigate the photodynamic sterilization efficiency of sodium chlorophyllin on Escherichia coli and Staphylococcus aureus;and the MTT method was used to determine the photodynamic antitumor effect of sodium chlorophyllin on rat glioma cell C6. [Results] The sterilization rates of sodium chlorophyllin with the 100 J/cm^2 photodynamic treatment were 98.96% 1.284 and 100% 0 on S. aureus and E. coli, respectively. The half maximal inhibitory concentration(IC50) of the sodium chlorophyllin photodynamic therapy with 5, 10 and 20 J/cm^2 on C6 were 69.9, 48.21 and 47.56 μg/ml respectively, and the dark toxicity was extremely low at 0 J/cm^2. [Conclusions]The photodynamic treatment mediated by the alcohol-extracted sodium chlorophyllin from Spirulina showed excellent inhibitory effects on bacteria and tumor cells. This study initially reveals its excellent photodynamic performance and provides a reference for its in-depth application in the field of photodynamic therapy.

关 键 词：Photodynamic technology SPIRULINA Sodium chlorophyllin Staphylococcus aureus Escherichia coli GLIOMA 

分 类 号：R454.2[医药卫生—治疗学]