Naa10p、TSGF及RUNx2在口腔黏膜癌前病变、口腔鳞癌中的应用  

Application of Naa10p,TSGF and RUNx2 in the oral mucosal precancerous lesions and oral squamous cell carcinoma

作　　者：刘春丽 王国杰 郑朝辉 曹瑞 LIU Chunli;WANG Guojie;ZHENG Chaohui;CAO Rui(Department of Stomatology,Second Affiliated Hospital of Xingtai Medical College,Xingtai,Hebei,China,054000;Department of Radiology,Xingtai Third Hospital,Xingtai,Hebei,China,054000)

机构地区：[1]邢台医专第二附属医院口腔科,河北邢台054000 [2]邢台市第三医院放射科,河北邢台054000

出　　处：《分子诊断与治疗杂志》2021年第1期12-16,共5页Journal of Molecular Diagnostics and Therapy

基　　金：邢台市科技计划项目(2017ZC044)。

摘　　要：目的分析N端α位乙酰基转移酶10蛋白(Naa10p)、肿瘤特异性生长因子(TSGF)及人侏儒相关转录因子2(RUNx2)联合检测在口腔黏膜癌前病变、口腔鳞癌(OSCC)诊断及鉴别诊断中的应用价值。方法选取2018年4月至2020年4月本院口腔科收治的65例OSCC患者,最终62例满足纳入标准,作为OSCC组。选取同期收治的50例癌前病变(扁平苔藓、白斑、红斑、慢性溃疡长期不愈)患者为癌前病变组,同时选取同期体检的40例健康者为正常对照组。收集所有研究对象临床资料,比较OSCC组、癌前病变组及正常对照组Naa10p、TSGF及RUNx2表达水平差异,分别对比Naa10p、TSGF及RUNx2单独及联合用于诊断OSCC的临床诊断性能(灵敏度、特异性及准确度);比较不同临床分期OSCC患者Naa10p、TSGF及RUNx2表达情况,采用ROC曲线分析Naa10p、TSGF、RUNx2单项及联合检测水平对OSCC的预测价值。结果三组Naa10p、TSGF水平及RUNx2阳性率比较:OSCC组>癌前病变组>正常对照组,差异具有统计学意义(P<0.05)。Naa10p、TSGF及RUNx2联合检测的敏感性、准确性均高于任何单一血清检测,差异有统计学意义(P<0.05)。Ⅲ、Ⅳ期Naa10p、TSGF水平及RUNx2阳性率高于Ⅰ、Ⅱ期患者,差异具有统计学意义(P<0.05)。采用ROC曲线分析Naa10p、TSGF、RUNx2单项及联合检测OSCC的预测价值,Naa10p、TSGF、RUNx2单项及联合检测曲线下面积分别为0.694、0.677、0.830、0.883,各指标曲线下面积以联合检测最大。结论Naa10p、TSGF及RUNx2表达在OSCC患者显著升高,且与患者临床分期密切相关,联合三者检测可作为预测OSCC发生的有效手段,临床上应重视三者水平的检测,进而改善患者预后。Objective To analyze the application value of combined detection of N-α-acetyltransferase 10 protein(Naa10p),Tumor Supplied Group of Factors(TSGF),and Runt-related Transcription Factor 2(RUNx2)in the diagnosis and differential diagnosis of oral mucosal precancerous lesions and oral squamous cell carcinoma(OSCC).Method 65 OSCC patients who were admitted to the Department of Stomatology in our hospital from April 2018 to April 2020 were selected.62 patients met the inclusion criteria and were recruited as the OSCC group.50 patients with precancerous lesions(lichen planus,leukoplakia,erythema,chronic ulcers that do not heal for a long time)were selected as the precancerous lesion group,and 40 normal subjects who received physical examination during the same period were selected as the health control group.The clinical data of all subjects were collected,and the differences in the expression levels of Naa10p,TSGF and RUNx2 in the OSCC group,precancerous lesion group and thecontrol group were measured and compared.The clinical diagnostic performance(sensitivity,specificity,and accuracy)of Naa10p,TSGF,RUNx2 alone and their combination to diagnose OSCC were compared,respectively.The expressions of Naa10p,TSGF and RUNx2 in OSCC patients at different clinical stages were compared,and the ROC curve was used to analyze the predictive value of Naa10p,TSGF,RUNx2 and the combined detection for OSCC.Results The levels of Naa10p,TSGF and RUNx2 positive rates in the three groups were compared:OSCC group>precancerous lesion group>normal control group,the difference was statistically significant(P<0.05).The sensitivity and accuracy of the combined detection of Naa10p,TSGF and RUNx2 were higher than those of any single serum detection,and the difference was statistically significant(P<0.05).The level of Naa10p,TSGF and the positive rate of RUNx2 in patients at the stageⅢandⅣwere higher than those in patients at the stageⅠandⅡ,and the difference was statistically significant(P<0.05).The areas under the Naa10p,TSGF,RUNx2 and

关 键 词：Naa10p TSGF RUNX2 口腔黏膜癌前病变 口腔鳞癌 

分 类 号：R739.8[医药卫生—肿瘤]