新型纳米材料与噬菌体展示技术在真菌毒素检测中的应用  

Application of new nanomaterials and phage display technology in mycotoxin detection

作　　者：冯林 陈雪岚[1] FENG Lin;CHEN Xuelan(School of Life Science,Jiangxi Normal University,Nanchang 330022,China)

机构地区：[1]江西师范大学生命科学学院,江西南昌330022

出　　处：《食品与发酵工业》2021年第3期230-236,共7页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31960014);国家自然科学基金项目(31660019)。

摘　　要：真菌毒素是真菌在生长繁殖过程中产生的次级有毒代谢产物,具有致癌、致畸、致突变的毒性作用,严重危害人体健康,因此对其检测与控制非常重要。现有检测方法普遍存在样品预处理复杂、成本高、操作繁琐等缺点,且会对操作人员和环境造成潜在二次危害。新型纳米材料可以提高真菌毒素的检测灵敏度,噬菌体展示技术可以筛选真菌毒素的模拟表位和抗真菌毒素的重组抗体,降低检测成本,达到无毒检测真菌毒素的目的。文章综述了近年来不同种类的纳米材料与噬菌体展示技术以及两者相互结合在真菌毒素检测方面的应用,并对其未来的发展进行了展望。Mycotoxins are toxic secondary metabolites produced by some fungi during growth and reproduction.They have carcinogenic,teratogenic and mutagenic effects,which seriously endanger human health.Therefore,their detection and control are of great importance.However,the existing detection methods generally have the disadvantages of complex sample pretreatment,high cost and cumbersome operation,and may cause potential secondary hazards to operators and the environment.The detection sensitivity of mycotoxins can be improved with new nanomaterials,and the mimic epitopes of mycotoxins and the recombinant antibodies against mycotoxins can be screened through phage display technology,hence reducing the detection cost and achieving the purpose of non-toxic detection.The recent application of different nanomaterials and phage display technology and their combination in mycotoxin detection were reviewed,and the future development is prospected.

关 键 词：真菌毒素 新型纳米材料 噬菌体展示技术 无毒检测 灵敏度 模拟表位 

分 类 号：TS207.5[轻工技术与工程—食品科学] TB383.1[轻工技术与工程—食品科学与工程]