版纳鱼螈皮肤分泌液的提取及其抑菌活性研究  

Study on Extraction and Antibacterial Activity from the Skin Secretion of Ichthyophis Bannanicus

作　　者：潘洁明 韦玉梅 何相莲 贝永建[1] 赖洁玲[1] PAN Jie-ming;WEI Yu-mei;HE Xiang-lian;BEI Yong-jian;LAI Jie-ling(Yulin Normal University,Guangxi Yulin 537000,China)

机构地区：[1]玉林师范学院,广西玉林537000

出　　处：《南宁师范大学学报（自然科学版）》2020年第4期102-106,共5页Journal of Nanning Normal University：Natural Science Edition

基　　金：玉林师范学院大学生创新创业训练计划项目(201810606033);2019年度广西高校中青年教师基础能力提升项目(2019KY0589);广西自然科学基金项目(青年科学基金)(2018GXNSFBA281098)。

摘　　要：为检测版纳鱼螈皮肤分泌液的抗菌活性,采用乙醚刺激法提取其皮肤分泌液,采用BCA法测定分泌液的蛋白浓度和SDS-PAGE凝胶电泳法检测分泌液中的蛋白分子量,并采用滤纸片扩散法检测抑菌活性。结果表明:版纳鱼螈皮肤分泌液对4种细菌和7种真菌病菌均无抑菌作用;分泌液的蛋白含量为3.08mg/mL,蛋白的分子量范围为170~10kDa。To detect the antibacterial activity of the skin secretion fromIchthyophis bannanicus.Skin secretions were obtained by ether stimulation,and the concentrations of protein were detected by the BCA protein quantification kit and the molecular weights of proteins from skin secretions were determined by SDS-PAGE electrophoresis.The antimicrobial activities were tracked by disk diffusion.The results showed that the skin secretion fromIchthyophis.bannanicus had no antibacterial effect on4 kinds of bacteria and 7 kinds of fungal pathogens.The protein content of secretion was 3.08 mg/mL,and the molecular weight range of protein was 170~10 kDa.

关 键 词：版纳鱼螈 皮肤分泌液 抑菌活性 SDS-PAGE凝胶电泳 

分 类 号：TS209[轻工技术与工程—食品科学]