超高效液相色谱-紫外检测法测定婴幼儿配方乳粉中的核苷酸含量  

Determination of Nucleotides in Infant Formula by Ultra-high Performance Liquid Chromatography

作　　者：王象欣 单艺 魏雪冬 李文斌[4] 鄂来明 姜毓君 WANG Xiangxin;SHAN Yi;WEI Xuedong;LI Wenbin;E Laiming;JIANG Yujun(Key Laboratory of Dairy Science,Ministry of Education,College of Food Science,Northeast Agricultural University,Harbin 150030,China;Heilongjiang Green Food Science Research Institute,Northeast Agricultural University,Harbin 150028,China;National Dairy Quality Supervision and Inspection Center,Harbin 150028,China;Dikma Technologies Inc.,Tianjin 300402,China)

机构地区：[1]东北农业大学食品学院,乳品科学教育部重点实验室,黑龙江哈尔滨150030 [2]东北农业大学黑龙江省绿色食品科学研究院,黑龙江哈尔滨150028 [3]国家乳制品质量监督检验中心,黑龙江哈尔滨150028 [4]天津迪科马实验设备有限公司,天津300402

出　　处：《食品科学》2021年第4期254-262,共9页Food Science

摘　　要：采用磺酸甜菜碱型两性离子亲水相互作用色谱法,建立婴幼儿配方乳粉中5种核苷酸(5’-胞嘧啶核苷酸、5’-尿嘧啶核苷酸、5’-腺嘌呤核苷酸、5’-次黄嘌呤核苷酸、5’-鸟嘌呤核苷酸)的测定方法。使用二维固相萃取模式,弱阴离子交换和强阳离子交换,对样品中的核苷酸进行提取,紫外检测器采集数据;用0.001%甲酸-乙腈溶液和pH 5的10 mmol/L磷酸二氢钠溶液作为流动相,等度洗脱。5种核苷酸在其线性范围内线性关系良好,定量限在0.10~0.15 mg/100 g范围内,方法回收率均在95%以上,精密度在10%以内。该方法快速、灵敏、准确,适合各类婴幼儿配方乳粉中核苷酸的测定。A method for the quantitation of nucleotides(cytidine 5’-monophosphate,uridine 5’-monophosphate,adenosine 5’-monophosphate,inosine 5’-monophosphate,and guanosine 5’-monophosphate)in infant formula was developed by sulfoalkyl betaine-based zwitterionic hydrophilic interaction liquid chromatography.The nucleotides in samples were extracted by two-dimensional solid-phase extraction with weak anion exchange and strong cation exchange,data acquisition was achieved using a UV detector.Chromatographic separation was performed by isocratic elution with a mobile phase consisting of 0.001%formic acid-acetonitrile and 10 mmol/L sodium dihydrogen phosphate solution at pH 5.Under the optimized conditions,good linearity was observed for these nucleotides,with a limit of quantification in the range of 0.10 to 0.15 mg/100 g.The method recovery was above 95%and the precision(expressed as relative standard deviation,RSD)was within 10%.This method was fast,sensitive and accurate and could be applied for the determination of nucleotides in infant formula samples.

关 键 词：核苷酸 磺酸甜菜碱型两性离子亲水相互作用色谱法 二维固相萃取 超高效液相色谱 婴幼儿配方乳粉 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]