NOTCH3 is expressed in human apical papilla and in subpopulations of stem cells isolated from the tissue  

作　　者：Mohamed Jamal Sami M.Chogle Sherif M.Karam George T-J.Huang 

机构地区：[1]Boston University,Henry M.Goldman School of Dental Medicine,Department of Endodontics,Boston,MA 02118,USA [2]United Arab Emirates University,Department of Anatomy,Faculty of Medicine and Health Sciences,Al-Ain,United Arab Emirates [3]University of Tennessee Health Science Center,College of Dentistry,Department of Bioscience Research,Memphis,TN 38163,USA [4]Current address:Department of Bioscience Research,College of Dentistry,University of Tennessee Health Science Center,875 Union Avenue,Memphis,TN 38163,USA

出　　处：《Genes & Diseases》2015年第3期261-267,共7页基因与疾病（英文）

基　　金：This work was supported in part by a grant from the Emirates Foundation e United Arab Emirates UAE University/NRF Grant and a grant from the National Institutes of Health RO1 DE019156(G.T.-J.H.).The authors deny any conflicts of interest.

摘　　要：NOTCH plays a role in regulating stem cell function and fate decision.It is involved in tooth development and injury repair.Information regarding NOTCH expression in human dental root apical papilla(AP)and its residing stem cells(SCAP)is limited.Here we investigated the expression of NOTCH3,its ligand JAG1,and mesenchymal stem cell markers CD146 and STRO-1 in the AP or in the primary cultures of SCAP isolated from AP.Our in situ immunostaining showed that in the AP NOTCH3 and CD146 were co-expressed and associated with blood vessels having NOTCH3 located more peripherally.In cultured SCAP,NOTCH3 and JAG1 were co-expressed.Flow cytometry analysis showed that 7%,16%and 98%of the isolated SCAP were positive for NOTCH3,STRO-1 and CD146,respectively with a rare 1.5%subpopulation of SCAP co-expressing all three markers.The expression level of NOTCH3 reduced when SCAP underwent osteogenic differentiation.Our findings are the first step towards defining the regulatory role of NOTCH3 in SCAP fate decision.

关 键 词：Apical papilla CD146 JAG1 NOTCH3 Osteogenesis SCAP STEMNESS STRO-1 

分 类 号：R74[医药卫生—神经病学与精神病学]