Combined treatment with valproic acid and estrogen has neuroprotective effects in ovariectomized mice with Alzheimer’s disease  

作　　者：Yan-Zhen Li Yuan-Jie Liu Wei Zhang Shi-Fang Luo Xin Zhou Gui-Qiong He 

机构地区：[1]Chongqing Key Laboratory of Neurobiology,Chongqing Medical University,Chongqing,China [2]Department of Anatomy,Chongqing Medical University,Chongqing,China [3]Department of Galactophore,Chongqing University Cancer Hospital,Chongqing,China

出　　处：《Neural Regeneration Research》2021年第10期2078-2085,共8页中国神经再生研究（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China,Nos.81671257,81371221,31600825(all to GQH).

摘　　要：Postmenopausal women with Alzheimer’s disease exhibit dramatically reduced sensitivity to estrogen replacement therapy,which is though to be related to an estrogen receptor(ER)α/ERβratio imbalance arising from a significantly decreased level of ERs of the brain.The aim of our study was to investigate whether valproic acid(VPA)can enhance the beneficial effects of estrogen on cognitive function through restoration of ERαand ERβexpression in the brain.We removed the ovaries of female APP/PS1 mice to simulate the low estrogen levels present in postmenopausal women and then administered VPA(30 mg/kg,intraperitoneal injection,once daily),17β-estradiol(E2)(2.4μg,intraperitoneal injection,once daily),liquiritigenin(LG)(50μg/kg,intragastric infusion,once daily),VPA+E2,or VPA+LG for 4 successive weeks.Compared with treatment with a single drug,treatment with VPA+E2 or VPA+LG significantly increased the level of glycogen synthase kinase 3β,increased the expression of estrogen receptorα,reduced the expression of small ubiquitin-like modifiers,and increased the level of estrogen receptorβ.This resulted in enhanced sensitivity to estrogen therapy,reduced amyloidβaggregation,reduced abnormal phosphorylation of the tau protein,reduced neuronal loss,increased dendritic spine and postsynaptic density,and significantly alleviated memory loss and learning impairment in Alzheimer’s disease.This study was approved by the Chongqing Medical University Animal Protection and Ethics Committee,China on March 6,2013.

关 键 词：17Β-ESTRADIOL amyloidβ dementia estrogen receptorα estrogen receptorβ glycogen synthase kinase-3β LIQUIRITIGENIN MENOPAUSE neuron loss tau 

分 类 号：R749.16[医药卫生—神经病学与精神病学]