Neural stem cell-based in vitro bioassay for the assessment of neurotoxic potential of water samples  

作　　者：Muhammad Irfan Masood Natalie Tamara Hauke Muhammad Jawad Nasim Muhammad Sarfraz Mahrukh Naseem Karl Herbert Schäfer 

机构地区：[1]Division of Bioorganic Chemistry,School of Pharmacy,Saarland University,D-66123 Saarbrücken,Germany [2]Applied Life Sciences,University of Applied Sciences Kaiserslautern,66482 Zweibrücken,Germany [3]ENS Group,University of Applied Sciences Kaiserslautern,66482 Zweibrücken,Germany [4]Abwasserverband Bergstrasse,Altau 10,69469 Weinheim,Germany [5]Institute of Pharmaceutical Sciences,University of Veterinary and Animal Sciences,54000 Lahore,Pakistan [6]Department of Zoology,University of Balochistan,Quetta 87550,Pakistan

出　　处：《Journal of Environmental Sciences》2021年第3期72-86,共15页环境科学学报（英文版）

基　　金：the Higher Education Commission(HEC),Pakistan(Ref.PD/OS-II/Batch-VI/Germany/2015/77159/17755);the University of Applied Sciences Kaiserslautern,Germany for providing financial support to execute the present project。

摘　　要：Intensive agriculture activities,industrialization and growing numbers of wastewater treatment plants along river banks collectively contribute to the elevated levels of neurotoxic pollutants in natural water reservoirs across Europe.We established an in vitro bioassay based upon neural stem cells isolated from the subventricular zone of the postnatal mouse to evaluate the neurotoxic potential of raw wastewater,treated sewage effluent,groundwater and drinking water.The toxic potential of water samples was evaluated employing viability,proliferation,differentiation and migration assays.We found that raw wastewater could reduce the viability and proliferation of neural stem cells,and decreased the neuronal and astrocyte differentiation,neuronal neurite growth,astrocyte growth and cell migration.Treated sewage water also showed inhibitory effects on cell proliferation and migration.Our results indicated that relatively high concentrations of nitrogenous substances,pesticides,mercuric compounds,bisphenol-A,and phthalates,along with some other pollutants in raw wastewater and treated sewage water,might be the reason for the neuroinhibitory effects of these water samples.Our model successfully predicted the neurotoxicity of water samples collected from different sources and also revealed that the incomplete removal of contaminants from wastewater can be problematic for the developing nervous system.The presented data also provides strong evidence that more effective treatments should be used to minimize the contamination of water before release into major water bodies which may be considered as water reservoirs for human usage in the future.

关 键 词：Neural stem cells Subventricular zone NEUROTOXICITY BIOASSAY Treated sewage effluents PESTICIDES 

分 类 号：X824[环境科学与工程—环境工程]