中肋骨条藻高效溶藻菌FDHY-C3的分离鉴定及溶藻作用研究  

Isolation and identification of a high efficiency algicidal bacterium FDHY-C3 and algicidal characteristics on Skeletonema costatum

作　　者：石新国 李悦 肖宇淳 郑文煌 刘乐冕 陈剑锋[1,2] SHI Xin-guo;LI Yue;XIAO Yu-chun;ZHENG Wen-huang;LIU Le-mian;CHEN Jian-feng(College of Biological Science and Engineering,Fuzhou University,Fuzhou 350108,China;Fujian Engineering Research Center for Comprehensive Utilization of Marine Products Waste,Fuzhou University,Fuzhou 350108,China)

机构地区：[1]福州大学生物科学与工程学院,福建福州350108 [2]海产品废弃物综合利用工程技术研究中心,福建福州350108

出　　处：《海洋环境科学》2021年第1期114-121,共8页Marine Environmental Science

基　　金：国家自然科学基金项目(41976130);福州市“十三五”海洋经济创新发展示范平台项目(FZHJ15)。

摘　　要：从福建长乐海域的表层海水中分离获得一株对中肋骨条藻具有高效溶藻作用的溶藻菌FDHYC3,通过形态学、生理生化及16S rDNA序列分析表明,菌株FDHY-C3属于交替单胞菌属(Alteromonas)。该菌株对常见的甲藻(dinoflagellate)、硅藻(diatom)以及针胞藻(raphidophyte)类的多种赤潮藻都有较高的溶藻作用,对中肋骨条藻溶藻效果尤为明显,72 h可达98.18%。溶藻菌FDHY-C3通过胞外分泌物间接溶藻;溶藻物质的溶藻特性不受反复冻融的影响,但是受酸碱性及温度的影响;溶藻活性物质具有被乙醇和乙酸乙酯沉淀的特性,为蛋白类物质,分子量为10~14 kD。An algicidal bacterium(FDHY-C3)with high-efficiency algicidal activity was isolated from the surface water of Changle coastal area,Fujian province.The morphological,physiological,biochemical and 16S rDNA sequence analysis was performed and the results indicated that the strain FDHY-C3 belongs to the genus of Alternaria.This strain showed a broad algicidal activity on diatoms,dinoflagellates and raphidophytes.The highest algicidal rate was detected on Skeletonema costatum,the algicidal rate can reach 98.18% in 72 h.The algicidal compounds was detected in extracellular secretions of FDHY-C3 cells through an indirectly dissolve way on target algal cells.The algal-lytic compounds of FDHY-C3 were not affected by freezing,but were affected by acidity and alkalinity and high temperature.The compounds can be extracted by ethanol and ethyl acetate,and was estimated to be protease.The molecular weight of this compound was between 10 kD and 14 kD.

关 键 词：赤潮 赤潮藻 中肋骨条藻 溶藻菌 溶藻特性 

分 类 号：X172[环境科学与工程—环境科学]