基于不同颜色山楂花MYB家族的挖掘与比较分析  

Excavation and Comparative Analysis of MYB Family Based on Hawthorn Flowers With Different Colors

作　　者：李戌彦 杨忠义[1,3,4] 纪薇 杨明霞[2,4] LI Xuyan;YANG Zhongyi;JI Wei;YANG Mingxia(College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801;Pomology Instltute,Shanxi Agricultural University,Taigu,Shanxi 030801;Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau,Ministry of Agriculture,Taiyuan,Shanxi 030031;Key Laboratory of fruit germplasm creation and Utilization in Shanxi Province,Taiyuan,Shanxi 030031)

机构地区：[1]山西农业大学园艺学院,山西太谷030801 [2]山西农业大学果树研究所,山西太谷030801 [3]农业部黄土高原作物基因资源与种质创制重点实验室,山西太原030031 [4]果树种质创制和利用山西省重点实验室,山西太原030031

出　　处：《核农学报》2021年第1期49-59,共11页Journal of Nuclear Agricultural Sciences

基　　金：山西省农业科学院博士基金(YBSJJ1810);山西省农业科学院生物育种工程(17yzgc041);山西省重点研发计划-中药现代化振东专项基金课题研究(2016ZD0109)。

摘　　要：为了研究MYB转录因子家族对山楂花色的影响,明确MYB转录因子在花色芽变品种中的表达情况,采用MYB蛋白质理化性质分析、motif结构预测、基因进化树分析以及基因表达量分析等方法对转录组数据进行深度挖掘。结果表明,共筛选出73个MYB转录因子,其中35个1R-MYB成员、36个2R-MYB成员、2个3R-MYB成员。1R-MYB蛋白序列的长度在50~786个氨基酸之间,2R-MYB蛋白序列长度在88~979个氨基酸之间,且仅有3个MYB基因家族蛋白为稳定蛋白。以拟南芥2R-MYB蛋白为参考进行系统进化树分析,19个山楂2R-MYB蛋白被分入14个已有一定研究基础的亚组中;1R-MYB蛋白进行单独分析,被分为6个亚组。通过差异表达分析,发现8个可能调控山楂花色的MYB转录因子。本研究结果为进一步研究影响山楂花色的MYB转录因子提供了一定的参考数据。In order to study the effect of MYB transcription factor family on the flower color of hawthorn,and to determine whether MYB transcription factors are differentially expressed in bud mutation varieties,the transcriptome were excavated by the analysis of the physical and chemical properties of MYB protein,motif structure prediction,phylogenetic tree analysis,and gene expression analysis.Totally 73 MYB transcription factors were screened,including 351 R-MYB members,362 R-MYB members,and two 3 R-MYB members.The length of 1 R-MYB proteins sequences are 50-786 amino acids,the length of 2 R-MYB proteins sequences are 88-979 amino acids,and only 3 MYB gene family proteins are stable proteins.The Arabidopsis 2 R-MYB proteins were used as a reference for phylogenetic tree analysis.The 19 hawthorn R2 R3-MYB proteins were classified into 14 subgroups with a certain research basis;the 1 R-MYB protein were analyzed separately and were divided into 6 subgroups.Through differential expression analysis,eight MYB transcription factors were found,which may regulate the color of hawthorn.The results of this study provide certain reference data for further research on MYB transcription factors that affect the flower color of hawthorn.

关 键 词：山楂 MYB转录因子 转录组 花色 

分 类 号：S661.5[农业科学—果树学]