基于高效液相色谱-气相色谱联用技术的矿物油分析研究进展  

Research Progress of Analysis of Mineral Oil Hydrocarbons Using On-Line High Performance Liquid Chromatography Coupled with Gas Chromatography

作　　者：武彦文[1] 李冰宁[1] 刘玲玲[1] 欧阳杰[2] WU Yan-Wen;LI Bing-Ning;LIU Ling-Ling;OUYANG Jie(Beijing Center for Physical&Chemical Analysis,Beijing Food Safety Analysis and Testing Engineering Research Center,Beijing 100094,China;Department of Food Science and Engineering,College of Biological Sciences and Technology,Beijing Forestry University,Beijing 100083 China)

机构地区：[1]北京市理化分析测试中心,北京市食品安全测试工程技术研究中心,北京100094 [2]北京林业大学生物科学与技术学院食品科学与工程系,北京100083

出　　处：《分析化学》2021年第3期341-349,共9页Chinese Journal of Analytical Chemistry

基　　金：北京市自然科学基金项目(No.2182020);北京市科学技术研究院改革与发展经费培育类项目(No.PY2020JC38)资助。

摘　　要：近年来,利用高效液相色谱-气相色谱联用技术(HPLC-GC)分析矿物油的研究发展迅速。矿物油源于石油与合成油,是涵盖一定碳数范围的碳氢化合物,主要分为烷烃和芳烃两大类。矿物油可通过多种途径迁移至食品和环境中,影响人类健康,然而其分析检测面临较大挑战。HPLC-GC(配备氢火焰离子化检测器)通过将净化、分离与检测相结合,很好地解决了矿物油分析中的选择性和灵敏度等问题。该技术的核心是通过保留间隙、Y形件接口和溶剂蒸气排出实现了GC大体积进样,将常规HPLC的洗脱流分全部转移至GC分析,将矿物油的分析灵敏度提高了两个数量级以上;同时避免了分析过程的污染引入,保证了分析的重现性和准确度。本文详细阐述了HPLC-GC的发展历程以及HPLC与GC分析条件的相互匹配,评述了基于HPLC-GC联用技术的干扰物去除与矿物油富集方法,并对HPLC-GC在矿物油分析中应用前景和发展趋势进行了展望。In the past decades,the application of on-line high performance liquid chromatography coupled with gas chromatography(HPLC-GC)has rapidly developed in quantitative analysis of mineral oil hydrocarbons(MOH).MOH are derived from petroleum and synthetic fuels and cover a large range of boiling points,mainly consisting of mineral oil saturated hydrocarbons(MOSH)and aromatic hydrocarbons(MOAH).Usually,MOH are migrated from variety of sources to various foods,thus existing in foods widely,which have been an adverse impact on human health.However,the analysis of MOH is challenging due to the limited selectivity and sensitivity of hydrogen flame ionization detector(FID).The on-line HPLC-GC combines high separation efficiency of HPLC and equal-carbon-response detection of GC-FID,which solves the problem of selectivity and sensitivity of MOH analysis.The innovation of retention gap,Y-piece interface and solvent vapor exit equipped in HPLC-GC employ large volume injection into GC-FID.Since the elution fractions from HPLC are completely transferred to GC,the analytical sensitivity of MOH is enhanced by more than two orders of magnitude and the contaminants introduced are minimized during the analytical procedure,ensuring reproducibility and accuracy of the MOH analysis.In this paper,the research history of interfaces used in on-line HPLC-GC and the matching conditions of HPLC and GC were described in detail and the sample preparation methods of interference removal and MOH enrichment based on HPLC-GC were reviewed.The limitations of HPLC-GC were summarized and the future application of the technology and the development trend in MOH analysis were prospected in the end.

关 键 词：高效液相色谱-气相色谱联用 矿物油 食品 分析 干扰物 评述 

分 类 号：O657.63[理学—分析化学] TE622.1[理学—化学]