高效液相色谱法测定婴幼儿营养米粉中维生素A、D_(3)和E含量的不确定度评定  

Evaluation of uncertainty in the determination of V_(A),VD_(3) and V_(E) in nutritional rice flour for infants and young children by HPLC

作　　者：张荣彬 戴志勇 李梦怡 陈振桂 任国谱[1] ZHANG Rong-bin;DAI Zhi-yong;LI Meng-yi;CHEN Zhen-gui;REN Guo-pu(Food Institute of Central South University of Forestry and Technology,Changsha,Hunan 410004,China;Engnice Holding Group Co.,Ltd.,Changsha,Hunan 410005,China)

机构地区：[1]中南林业科技大学食品科学与工程学院,湖南长沙410004 [2]英氏控股集团股份有限公司,湖南长沙410005

出　　处：《食品与机械》2021年第2期85-92,共8页Food and Machinery

摘　　要：依据JF1059.1—2012《测量不确定度评定与表示》,建立高效液相色谱法同时测定婴幼儿营养米粉中维生素A、D_(3)和E含量不确定度的数学模型,对测量过程中的各不确定度来源进行逐层分析、量化和合成。当取样量为5.000 g时,样品中维生素A、D_(3)和E扩展不确定度为0.1884,0.0093,0.0067μg/g,测定含量为(9.2500±0.1884),(0.1140±0.0093),(0.1815±0.0067)μg/g。最终结果的不确定度主要是由标准溶液储备液配制、标准工作曲线拟合和回收率引起。The uncertainty of the determination of V_(A),VD_(3) and V_(E) content in infant nutritional rice flour by HPLC was evaluated.According to JF1059.1—2012"evaluation and Representation of Measurement Uncertainty",a mathematical model for simultaneous determination of V_(A),VD_(3) and V_(E) content uncertainty in infant nutrition rice flour by HPLC was established,and the sources of uncertainty in the measurement process were analyzed,quantified and synthesized layer by layer.When the sampling volume was 5.000 g,the expanded uncertainty of V_(A),VD_(3) and V_(E) in the sample was 0.1884,0.0093,0.0067μg/g,and the measured content was(9.2500±0.1884),(0.1140±0.0093),(0.1815±0.0067)μg/g,respectively.The uncertainty of the final result was mainly caused by the preparation of standard solution reserve,fitting of standard working curve and recovery rate.

关 键 词：高效液相色谱 婴幼儿营养米粉 维生素A 维生素D_(3) 维生素E 不确定度评定 

分 类 号：O657.72[理学—分析化学] TS216[理学—化学]