婴幼儿配方奶粉对人结肠腺癌系Caco-2细胞和SD大鼠腹腔巨噬细胞自由基水平的影响  

Effects of Infant Formula Milk Powder on Free Radical Levels in Human Colon Adenocarcinoma Line Caco-2 Cells and SD Rat Peritoneal Macrophages

作　　者：丁亚男 高观祯[1] 汪惠勤[1] 周建武[1] 饶平凡[1] Ding Yanan;Gao Guanzhen;Wang Huiqin;Zhou Jianwu;Rao Pingfan(Shanghai Institutes for Biological Sciences of Chinese Academy of Sciences-Zhejiang Gongshang University Joint Centre for Food and Nutrition Research,Hangzhou 310035)

机构地区：[1]中国科学院上海生命科学研究院-浙江工商大学食品营养科学联合研究中心,杭州310035

出　　处：《中国食品学报》2021年第2期9-17,共9页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2016YFD0400202)。

摘　　要：为探究婴幼儿配方奶粉对消化道上皮细胞及黏膜免疫系统自由基水平调节能力的影响,随机选取5种市售一段婴幼儿配方奶粉,以SD大鼠腹腔巨噬细胞和人结肠腺癌系Caco-2细胞为检测模型,测定配方奶粉对细胞增值率影响的质量浓度范围,并采用经部分修改的细胞内抗氧化活性测定方法(CAA)评价配方奶粉的胞内抗氧化能力。在无外源性自由基诱导剂作用下,检测配方奶粉对2种细胞胞质及线粒体内活性氧自由基水平的影响。研究结果显示:在质量浓度40~1000μg/mL范围,配方奶粉对细胞增殖均无影响。在2种细胞的CAA模型中,受测的5种配方奶粉均表现出胞内抗氧化活性,然而CAA值存在明显差异。在无外源自由基诱导剂AAPH作用时,3种配方奶粉对胞浆及线粒体内活性氧自由基表现出清除作用,另外2种配方奶粉则上调了自由基水平。To reveal the cellular antioxidant capacity of infant formula milk powder on digestive tract epithelial cells and its mucosal immune cells,five infant formula products were randomly selected from the market and subjected to the cellular models based on rat peritoneal macrophage and human colon adenocarcinoma line Caco-2 cells for determination of their cytotoxic or proliferative effects.A modified Cellular Antioxidant Activity Assay(CAA)method was used to evaluate the cellular antioxidant capacity of the products.Meanwhile,in the absence of exogenous free radical inducer AAPH,the influences of the products on the level of free radicals in cytosol and mitochondria were also investigated,respectively.The results showed that the products of infant formula exhibited neither cytotoxic effects nor proliferative effects against these two cell lines in the concentration range of 40-1000μg/mL.Five products showed cellular antioxidant activity in CAA models in both cell lines,but varied obviously in CAA values.In the absence of AAPH,three products showed scavenging effect on the free radicals within cytosol and mitochondrial,the other two,instead,increased the free radical levels within cytosol and mitochondrial.

关 键 词：婴幼儿配方奶粉 胞内抗氧化模型 氧化应激 抗氧化能力 

分 类 号：TS252.51[轻工技术与工程—农产品加工及贮藏工程]