藻蓝色素调控RIPK1对多种非小细胞肺癌细胞活性的影响  

Effects of Phycocyanin Regulating RIPK1 on the Activities of Multiple Non-small Cell Lung Cancer Cells

作　　者：郝帅[1] 李凡念 刘媛璞 李爽[1] 杨起 李前程 王成涛[1] Hao Shuai;Li Fannian;Liu Yuanpu;Li Shuang;Yang Qi;Li Qiancheng;Wang Chengtao(Beijing Engineering and Technology Research Center of Food Additives,School of Food and Health,Beijing Technology and Business University,Beijing 100048)

机构地区：[1]北京市食品添加剂工程技术研究中心北京工商大学食品与健康学院,北京100048

出　　处：《中国食品学报》2021年第2期18-27,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金面上项目(32072231)。

摘　　要：藻蓝蛋白又称藻蓝色素蛋白(PC),是一种具有抗肿瘤活性的天然食品着色剂,对包括肺癌在内的多种肿瘤具有显著的抑制作用,然而其在非小细胞肺癌(NSCLC)中的调控机理尚不完全明确。本研究以H1299、H460和LTEP-a-23种典型的非小细胞肺癌细胞为模型,探究藻蓝蛋白在抑制非小细胞肺癌增殖和诱导细胞凋亡过程中的关键调控机制。细胞表型试验结果证实:藻蓝蛋白能够显著抑制3种细胞的体外增殖能力,并通过调控Bax和Bcl-2诱导细胞凋亡。利用转录组技术对H1299细胞进行测序,受体相互作用蛋白激酶1(RIPK1)经藻蓝蛋白处理后出现极显著下调。对3种细胞转染siRNA特异性沉默RIPK1的表达后,细胞的体外增殖能力也明显降低,同时诱导细胞凋亡,与藻蓝蛋白处理后的表型结果一致。研究结果表明:藻蓝蛋白通过下调RIPK1的表达而抑制H1299、H460和LTEP-a-2非小细胞肺癌的体外增殖并诱导细胞凋亡,这为天然功能性食用色素的开发和利用以及非小细胞肺癌的安全性治疗奠定了理论基础。Phycocyanin(PC)is a kind of natural food colorant with anti-cancer activity.Phycocyanin exerts significant inhibition effects on multiple tumors including lung cancer.However,the potential regulatory mechanism of phycocyanin in non-small cell lung cancer(NSCLC)cells is still unknown.In this study,we used three types of NSCLC cells(H1299,H460 and LTEP-a-2)as research model,and aimed to investigate the anti-proliferation and pro-apoptosis mechanism of phycocyanin in these cells.Cell phenotype experimental result indicated that phycocyanin could significantly inhibit the proliferation and induce apoptosis of H122,H460 and LTEP-a-2 cells.Transcriptome sequencing analysis on H1299 cells suggested that the expression of receptor interacting proteinkinase1(RIPK1)significantly downregulated after phycocyanin treatment.Knock-down expression of RIPK1 using siRNA could also decrease the proliferation and induce the apoptosis of H1299,H460 and LTEP-a-2 cells,which was consist with the phenotype results of phycocyanin.This study indicated that phycocyanin could inhibit proliferation and induce apoptosis of multiple non-small cell lung cancer cells through down-regulating RIPK1,which provided important theoretical basis for the development and utilization of natural functional food additives,also provided useful information on the treatment of non-small cell lung cancer.

关 键 词：非小细胞肺癌 藻蓝蛋白 受体相互作用蛋白激酶1(RIPK1) 细胞增殖 细胞凋亡 

分 类 号：R734.2[医药卫生—肿瘤] TS202.3[医药卫生—临床医学]