石榴皮提取物改善肥胖大鼠糖、脂代谢紊乱的分子机制  

Molecular Mechanism of Pomegranate Peel Extract Improving Glucose and Lipid Metabolism Disorder in Obese Rats

作　　者：张飒乐[1] 魏香兰 李英[1] 李晓明[1] 方欢乐[1] Zhang Sale;Wei Xianglan;Li Ying;Li Xiaoming;Fang Huanle(Medical College of Xi’an Peihua University,Xi’an 710125;Pharmacy Department of Xi’an Chest Hospital,Xi’an 710100)

机构地区：[1]西安培华学院医学院,西安710125 [2]西安市胸科医院药剂科,西安710100

出　　处：《中国食品学报》2021年第2期109-115,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(81402924);西安市科技计划项目【SF1509(2)】。

摘　　要：目的:探讨高脂饮食诱发的大鼠肥胖对肝脏糖代谢的影响,揭示石榴皮提取物(EPP)改善肥胖大鼠糖、脂代谢紊乱的分子机制。方法:应用高脂饲料(D12451)和对照饲料(D12450H)分别饲养大鼠16周,复制肥胖动物模型。在造模的同时,灌胃大鼠3个剂量的EPP(50,100,200 mg/kg)进行干预。结果:高脂饲料喂养16周后大鼠体重、Lee’s指数和肝重指数显著增高;血清TC、TG、LDL-C和FFA水平增高,HDL-C水平降低;大剂量EPP干预可显著改善高脂大鼠体重、Lee’s指数、肝重指数和血脂水平,表明EPP可有效降低高脂饮食诱发的大鼠肥胖。EPP可显著改善肥胖大鼠肝脏显微结构和肝功能损伤。大鼠胰岛素抵抗和肝脏糖代谢的检测结果表明:高脂饮食大鼠存在明显的血糖增高和胰岛素抵抗现象,大剂量EPP可显著性降低血糖,改善胰岛素抵抗,提高肥胖大鼠肝脏组织IRS-2和GLUT2的蛋白水平。结论:EPP通过降低肥胖大鼠血脂和改善肝脏葡萄糖代谢紊乱发挥肝脏保护作用。Objective:To explore the effect of obesity induced by high fat diet on liver glycometabolism,and to reveal the molecular mechanism of pomegranate peel extract(EPP)in improving the disorder of glycometabolism and lipid metabolism in obese rats.Methods:Rats were fed with high-fat diet(D12451)and control diet(D12450H)for 16 weeks to replicate the obese animal model.Three doses of EPP(50,100,200 mg/kg)were administered by intragastric administration simultaneously with the model.Result:After 16 weeks of feeding with high fat diet,the body weight,Lee's index and liver weight index of rats increased,the levels of serum TC,TG,LDL-C and FFA increased,and the levels of HDL-C decreased.High dose of EPP could significantly improve the body weight,Lee’s index,liver weight index and blood lipid level of rats with high fat diet.It showed that EPP could effectively reduce the obesity induced by high-fat diet.At the same time,the results showed that EPP significantly reduced the liver microstructure and liver function damage of fat rats induced by fat.The detection of insulin resistance and hepatic glucose metabolism in rats showed that there was significant blood glucose increase and insulin resistance in rats with high-fat diet.High-dose EPP could significantly reduce blood glucose and improve insulin resistance.And EPP could increase liver tissue IRS-2 and GLUT2 protein levels.Conclusion:EPP exerted liver protection by reducing blood lipids in obese rats and improving hepatic glucose metabolism disorders.

关 键 词：糖、脂代谢紊乱 非酒精性脂肪肝 高脂饮食 石榴皮提取物 

分 类 号：R589.2[医药卫生—内分泌] TS209[医药卫生—内科学]