河蚬汤食源纳米颗粒对非酒精性脂肪肝长爪沙鼠的影响  

Effects of Freshwater Clam Soup Nanoparticles on Non-alcoholic Fatty Liver Disease of Meriones unguieulataus

作　　者：余兆硕 汪惠勤[1] 高观祯[1] 柯李晶[1] 周建武[1] 饶平凡[1] 余陈欢 Yu Zhaoshuo;Wang Huiqin;Gao Guanzhen;Ke Lijing;Zhou Jianwu;Rao Pingfan;Yu Chenhuan(SIBS.CAS-Zhejiang Gongshang University Joint Centre for Food and Nutrition Research,Hangzhou 310035;Laboratory Animal Center,Institute of Cancer and Basic Medicine,Chinese Academy of Sciences,Hangzhou 310018)

机构地区：[1]中国科学院上海生命科学研究院-浙江工商大学食品营养科学联合研究中心,杭州310035 [2]中国科学院肿瘤与基础医学研究所实验动物中心,杭州310018

出　　处：《中国食品学报》2021年第2期116-120,共5页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划项目(2016YFD0400202)。

摘　　要：通过构建非酒精性脂肪肝长爪沙鼠模型,探讨河蚬汤食源纳米颗粒(FNPs)对高脂长爪沙鼠的非酒精性脂肪肝的改善作用。结果显示:连续喂养高脂饲料4周后,高脂组长爪沙鼠肝脏TC、TG及AST与ALT水平均显著升高(P﹤0.05),经组织切片染色发现,高脂组肝组织呈现灶状坏死情况,说明非酒精性脂肪肝长爪沙鼠模型建立成功。与高脂模型组相比,通过口服或者灌胃河蚬汤FNPs的非酒精性脂肪肝长爪沙鼠的各项指标降低,其中血清TC与肝脏TC、AST水平均极显著降低(P﹤0.01),且肝脏各项指标与正常对照组无显著差异,说明无论口服还是灌胃方式喂养河蚬汤FNPs都具有降血脂及改善非酒精性脂肪肝的作用。此外,口服组最为接近正常组并优于灌胃组,这可能是口服喂养河蚬汤FNPs直接作用于消化道黏膜,从而发挥更强的护肝活性。This paper established a model of non-alcoholic fatty liver in Meriones unguieulataus(MU),aiming to explore the effect of food derived nanoparticles(FNPs)from freshwater clam soup on non-alcoholic fatty liver disease(NAFLD).Results showed that the levels of TC,TG,AST and ALT of MU exposed to high-fat diet for 4 weeks significantly rose(P﹤0.05).Besides,the liver tissue of the high-fat group showed focal necrosis,demonstrating the successful modeling of the MU with NAFLD.Compared with the high-fat model group,the indexes of the MU fed with oral or gavage administration of FNPs decreased,among which TC in serum and liver and AST in the liver slumped(P﹤0.01).The indexes in the liver were close to those of the normal control group.It suggested that the soup nanoparticles,whether oral or gastric administration for MU could effectively reduce hematic fat and intervene with nonalcoholic fatty liver disease.In addition,the effect of oral group was closer to normal and superior to gavage group.Oral administration may bestow the food nanoparticles the opportunity to directly interact with the digestive tract mucosa,so as to play a strong biological activity of hepatocyte protection.

关 键 词：河蚬汤 食源纳米颗粒 长爪沙鼠 非酒精性脂肪肝 食道黏膜 

分 类 号：R575.5[医药卫生—消化系统] TS254.1[医药卫生—内科学]