茯苓提取物参与灰树花液态深层发酵及对其活性物质产量的影响  

Poria cocos extract affected yield of bioactive constituents in submerged culture of Grifola frondosa

作　　者：吴力亚 吴天祥 汪玲 WU Liya;WU Tianxiang;WANG Ling(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;Guizhou Vocational College of Foodstuff Engineering,Guiyang 550025,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州食品工程职业学院,贵州贵阳550025

出　　处：《食品与发酵工业》2021年第5期57-62,共6页Food and Fermentation Industries

基　　金：国家自然科学基金项目(31460537)。

摘　　要：中药茯苓提取物参与灰树花液态深层共发酵,进而探究不同溶剂提取的、不同添加比例的茯苓提取物对灰树花活性物质产量的影响。结果表明,添加5 g/L的茯苓醇提液和7 g/L的茯苓水提液时,均能够提高灰树花的生物量及多糖、总黄酮、蛋白的含量。相比于空白对照组,在最适醇提物和水提物添加量下的菌丝体生物量分别提高了24.28%和29.04%,具有显著促进作用(P<0.05)。5 g/L醇提物添加条件下的胞外多糖、胞内多糖、胞外黄酮、胞内黄酮、胞外蛋白、胞内蛋白组与空白组相比具有显著促进作用(P<0.05),分别提高了17.8%、30.29%、8.32%、10.13%、10.49%和10.24%。7 g/L水提液添加条件下胞内多糖、胞外黄酮、胞内黄酮、胞外蛋白、胞内蛋白与空白组相比具有显著促进作用(P<0.05),分别提高了19.8%、3.16%、9.29%、10.24%和8.78%。表明添加一定量的茯苓提取物能够在一定程度上增加灰树花菌丝体的生长和活性代谢产物的含量,并且对多糖的产量影响尤为明显。The traditional Chinese medicine Poria cocos and Grifola frondosa were co-fermented in submerged fermentation.The effects of different solvent extraction and addition ratios of P.cocos extract on the yield of bioactive constituents of G.frondosa were investigated.The results showed that 5 g/L alcohol extract of P.cocos or 7 g/L water extract of P.cocos significantly increased the biomass,extracellular polysaccharides,intracellular polysaccharides,total flavonoids and protein of G.frondosa.Compared with the control group,the biomass of mycelium increased by 24.28%and 29.04%respectively under the optimal addition ratios of P.cocos extract,which had a significant promotion(P<0.05);the extracellular polysaccharides,intracellular polysaccharides,extracellular flavonoids,intracellular flavonoids,extracellular proteins,and intracellular proteins of G.frondosa increased by 17.8%,30.29%,8.32%,10.13%,10.49%,and 10.24%respectively when adding 5 g/L alcohol extract,which have a significant promotion(P<0.05);intracellular polysaccharides,extracellular flavonoids,intracellular flavonoids,extracellular proteins,and intracellular proteins of G.frondosa increased by 19.8%,3.16%,9.29%,10.24%,and 8.78%respectively with the addition of 7 g/L water extract of P.cocos,compared with the control group,that has a significant promotion(P<0.05).The investigation revealed that a certain concentration of P.coco s extract can significantly promote the biomass of G.frondosa mycelium,the yield of bioactive constituents,and especially the yield of G.frondosa polysaccharides.

关 键 词：灰树花 液态发酵 茯苓 代谢产物:活性成分 

分 类 号：TS201.3[轻工技术与工程—食品科学]