基于UPLC-Q-TOF/MS的黑灵芝多糖对大鼠急性肠道炎症作用机制研究  

Effect of Ganoderma lucidum Polysaccharides on Intestinal Inflammation in Rats Based on UPLC-Q-TOF/MS

作　　者：李露 付王威 吴睿婷 吴文英 尹术华 宋也好 万敏 李文娟 LI Lu;FU Wangwei;WU Ruiting;WU Wenying;YIN Shuhua;SONG Yehao;WAN Min;LI Wenjuan(College of Food Science,Nanchang University,Nanchang 330047,China;Food Inspection and Testing Institute of Jiangxi Province,Nanchang 330001,China)

机构地区：[1]南昌大学食品学院,江西南昌330047 [2]江西省食品检验检测研究院,江西南昌330001

出　　处：《食品工业科技》2021年第6期310-317,共8页Science and Technology of Food Industry

基　　金：国家自然科学基金地区项目(81860713);南昌大学研究生创新项目(CX2018116)。

摘　　要：探究黑灵芝多糖(PSG)对脂多糖(LPS)诱导的大鼠急性肠道炎症的作用机制。本实验将大鼠随机分为正常组(Con)、模型组(LPS)、阳性对照组(DEX)和黑灵芝多糖低、中、高剂量组(PSG),每组10只。PSG低、中、高剂量组分别灌胃黑灵芝多糖20、40、80 mg/kg。Con组、LPS组与PSG组则每天灌胃等量的生理盐水,连续灌胃7 d。第7 d灌胃结束后,腹腔注射0.8 mg/kg的LPS溶液建立急性肠道炎症模型。通过观察大鼠空肠组织形态、检测细胞因子IL-1β及IL-10含量等指标,结果发现,PSG对LPS诱导的大鼠具有抗炎作用。与LPS组相比,PSG组空肠组织形态明显得到改善,肠道组织中IL-1β和IL-10含量被调节至正常水平。进一步,利用超高效液相色谱-四级杆串联飞行时间质谱(UPLC-Q-TOF/MS)方法获得了四组大鼠盲肠内容物样品的代谢轮廓图。结果发现,与Con组相比,LPS组大鼠盲肠内容物中存在22种代谢物。同时,PSG干预可回调PC(24∶0/20∶4(5Z,8Z,11Z,14Z))、DG(16∶0/20∶3(5Z,8Z,11Z)/0∶0)等12种差异代谢物,进而影响甘油磷脂代谢、亚油酸代谢和α-亚麻酸代谢等三条代谢通路,发挥其对LPS诱导大鼠的抗炎作用。综上,PSG可改善LPS所致急性肠道炎症,作用机理与其抑制促炎因子的释放和代谢紊乱有关。The protective effects of Ganoderma atrum polysaccharide(PSG)on acute intestinal inflammation caused by lipopolysaccharide(LPS)were investigated.Rats were randomly divided into a control group(Con),a model group(LPS),positive control group(DEX)and three groups treated with PSG:Low-dose group(PSG-20),middle-dose group(PSG-40)and high-dose group(PSG-80),with 10 rats in each group.Rats were orally administered either vehicle(0.9%saline)alone or vehicle composition containing PSG(20,40,and 80 mg/kg/d)for 7 days.After intragastric administration on the 7th day,0.8 mg/kg LPS was injected intraperitoneally to establish an acute intestinal inflammation model.The results indicated that PSG has an anti-inflammatory effect on LPS-induced rats by observing jejunum histology,detecting the content of IL-1βand IL-10 in intestinal tissue.Compared with LPS group,the morphology of the jejunum tissue and the content of IL-1βand IL-10 were adjusted to normal levels.Furthermore,the metabolic effects of PSG were evaluated by screening and identifying differential metabolites and exploring metabolic pathways in rats based on UPLC-Q-TOF/MS metabolomics analysis.Accordingly,results indicated that 22 metabolites were significantly(P<0.05)changed in acute intestinal inflammation rats when compared to control rats.Meanwhile,PSG pretreatment could restore 12 different metabolites such as PC(24∶0/20∶4(5Z,8Z,11Z,14Z)),DG(16∶0/20∶3(5Z,8Z,11Z)/0∶0),and then participated in three metabolic pathways of glycerophospholipid metabolism,linoleic acid metabolism andα-linolenic acid metabolism,exerting its anti-inflammatory effects on LPS-induced rats.Altogether,PSG could improve LPS-induced acute intestinal inflammation and its mechanism were linked to inhibit release of proinflammatory mediators and reverse metabolic pathway disturbances.

关 键 词：黑灵芝多糖 急性肠道炎症 超高效液相色谱-四级杆串联飞行时间质谱 代谢组学 抗炎 

分 类 号：TS207.3[轻工技术与工程—食品科学]