重组胶原蛋白的绿色生物制造及其应用  

Green biological manufacture and application of recombinant collagen

作　　者：李阳 朱晨辉[1,2,3] 范代娣[1,2,3] LI Yang;ZHU Chenhui;FAN Daidi(Shaanxi Key Laboratory of Degradable Biomedical Materials,School of Chemical Engineering,Northwest University,Xi’an 710069,Shaanxi,China;Shaanxi R&D Center of Biomaterials and Fermentation Engineering,School of Chemical Engineering,Northwest University,Xi’an 710069,Shaanxi,China;Biotech.&Biomed.Research Institute,Northwest University,Xi’an 710069,Shaanxi,China)

机构地区：[1]陕西省可降解生物医用材料重点实验室,西北大学化工学院,陕西西安710069 [2]陕西省生物材料与发酵工程技术研究中心,西北大学化工学院,陕西西安710069 [3]西北大学生物医药研究院,陕西西安710069

出　　处：《化工进展》2021年第3期1262-1275,共14页Chemical Industry and Engineering Progress

基　　金：国家重点研发计划(2019YFA0905200);国家自然科学基金重点项目(21838009)。

摘　　要：胶原蛋白存在于各个组织器官,与动物胶原蛋白相比,重组胶原蛋白组分单一、安全性高、生产过程可控。本篇综述简述了重组胶原蛋白不同表达体系的构建,包括动植物以及微生物表达体系,比较了不同体系的优缺点。着重介绍了微生物体系中影响产物表达的不同发酵参数的调控,产物的分离纯化工艺以及重组胶原蛋白在医学领域的应用。提出微生物发酵体系较动植物体系成本低,操作简单,易于扩大生产;温度、pH、溶解氧、葡萄糖、乙酸浓度等影响大肠杆菌发酵中的蛋白表达量;酵母发酵中,甲醇添加量、温度、pH和溶解氧是主要影响参数;微生物发酵体系均需通过不同的粗纯及精纯技术获得纯度较高的产物。同时,重组胶原蛋白在生物医学领域发挥着重要作用。Collagen exists in all tissues and organs.Compared with animal collagen,recombinant collagen has a more single component,higher safety,and more controllable production process.This review briefly introduces the construction of different expression systems of recombinant collagen,including the expression systems of animals,plants,and microorganisms,and their advantages and disadvantages are compared.The regulation of different fermentation parameters affecting the expression of the product in the microbial system,the separation and purification process of the product,and the application of recombinant collagen in the medical field were introduced emphatically.It is pointed out that the microbial fermentation system has a lower cost,simple operation,and easier to expand production than the animal and plant system.Temperature,pH,dissolved oxygen,glucose,and acetic acid concentration affect the protein expression in Escherichia coli fermentation.In yeast fermentation,the addition amount of methanol,temperature,pH,and dissolved oxygen are the main influencing parameters.Microbial fermentation systems need to obtain high purity products through different crude and precision technologies.Meanwhile,recombinant collagen plays an important role in the field of biomedicine.

关 键 词：重组胶原蛋白 大肠杆菌 酵母 发酵 纯化 应用 

分 类 号：Q815[生物学—生物工程]