MAPKs信号通路在内皮素-1诱导的牙周膜干细胞成骨分化过程中的作用研究  

Effects of MAPKs Signaling Pathway in Osteogenic Differentiation of Periodontal Ligament Stem Cells Induced by Endothelin-1

作　　者：梁莉[1] 许亦权[1] 杨楠[1] 王辰 LIANG Li;XU Yi-quan;YANG Nan;WANG Chen(Department of Stomatology,the Eighth Medical Center,Chinese PLA General Hospital,Beijing 100091,China)

机构地区：[1]解放军总医院第八医学中心口腔科,北京100091

出　　处：《中国美容医学》2021年第2期96-98,共3页Chinese Journal of Aesthetic Medicine

摘　　要：目的:研究丝裂原激活蛋白激酶(Mitogen-activated protein kinases,MAPKs)信号通路在内皮素-1(Endothelin-1,ET-1)诱导的牙周膜干细胞|(Periodontal ligament stem cells,PDLSCs)成骨分化中的调控机制。方法:体外培养牙周膜干细胞,于ET-1处理前分别加入ERK通路抑制剂PD98059,JNK通路抑制剂SP600125,p38 a通路抑制剂SB203580.以未作任何处理的牙周膜干细胞为空白对照,以单独加入抑制剂的牙周膜干细胞为阴性对照,用100nM BT-1干预不同组牙周膜干细胞14d,通过实时定量PCR及Western blot技术检测细胞Runx2和0CN基因和蛋白的表达情况。结果:ET-1对牙周膜干细胞Runx2和0CN基因及蛋白表达具有显著促进作用,但PD98059抑制了ET-1对牙周膜干细胞Runx2和0CN表达的促进效应,而SP600125和SB203580的抑制作用不显著。结论:BT-1通过ERK信号通路调控牙周膜干细胞Runx2和0CN的表达。Objective To investigate the role of mitogen activated protein kinases(MAPKs)pathway on endothelin-1-induced osteogenic differentiation of periodontal ligament stem cells(PDLSCs).Methods The PDLSCs were stimulated with endothelin-1(ET-1),ERK pathway inhibitor PD98059,JNK pathway inhibitor SP600125 and p38αpathway inhibitor SB203580 for 14 days,respectively.The untreated PDLSCs served as normal control and the PDLSCs with inhibitor alone served as negative control.The expression of Runx2 and OCN mRNAs and proteins in PDLSCs were detected by real-time PCR and western blot,respectively.Results ET-1 significantly promoted the expression of Runx2 and OCN mRNAs and proteins in PDLSCs.PD98059 inhibited the effect of endothelin-1 on the expression of Runx2 and OCN in PDLSCs.However the inhibition of SP600125 and SB203580 was not significant.Conclusion These results indicate that ET-1 may modulate the expression of Runx2 and OCN via ERK signaling pathway in PDLSCs.

关 键 词：牙周膜干细胞 内皮素-1 丝裂原激活蛋白激酶 RUNX2 骨钙素 牙周炎 

分 类 号：R781.4[医药卫生—口腔医学]