Influence of three different anesthesia protocols on aged rat brain:a resting-state functional magnetic resonance imaging study  

作　　者：Yang Liu Hui-Qun Fu Yan Wu Zun-Shu Du Bo-Ran Li Xin Gao Guan-Wen Lin Shu-Yi Yang Tian-Long Wang 

机构地区：[1]Department of Anesthesiology,Xuanwu Hospital,Capital Medical University,Beijing 100053,China [2]Department of Anatomy,Capital Medical University,Beijing 100069,China

出　　处：《Chinese Medical Journal》2021年第3期344-352,共9页中华医学杂志（英文版）

基　　金：Beijing municipal administration of hospitals clinical medicine development of special funding support(Code:ZYLX201808);Beijing Municipal Health Commission(Code:Jing2019-2)。

摘　　要：Background:Resting-state functional magnetic resonance imaging(rs-fMRI)is a promising method for the study of brain function.Typically,rs-fMRI is performed on anesthetized animals.Although different functional connectivity(FC)in various anesthetics on whole brain have been studied,few studies have focused on different FC in the aged brain.Here,we measured FC under three commonly used anesthesia methods and analyzed data to determine if the FC in whole brain analysis were similar among groups.Methods:Twenty-four male aged Wistar rats were randomly divided into three groups(n=8 in each group).Anesthesia was performed under either isoflurane(ISO),combined ISO+dexmedetomidine(DEX)orα-chloralose(AC)according to the groups.Data of rs-fMRI was analyzed by FC in a voxel-wise way.Differences in the FC maps between the groups were analyzed by one-way analysis of variance andpost hoc two-samplet tests.Results:Compared with ISO+DEX anesthesia,ISO anesthesia caused increased FC in posterior brain and decreased FC in the middle brain of the aged rat.AC anesthesia caused global suppression as no increase in FC was observed.Conclusion:ISO could be used as a substitute for ISO+DEX in rat default mode network studies if the left temporal association cortex is not considered important.

关 键 词：ANESTHETICS Aged RAT Functional connectivity Magnetic resonance imaging 

分 类 号：R614[医药卫生—麻醉学]