Woodchuck hepatitis post-transcriptional regulatory element improves transgene expression and stability mediated by episomal vectors in CHO-K1 cells  

作　　者：Xiaoyin Wang Weili Zhang Yanlong Jia Meng Wang Dandan Yi Tian-yun Wang 

机构地区：[1]Department of Biochemistry and Molecular Biology,Xinxiang Medical University,Xinxiang 453003,China [2]International Joint Research Laboratory for Recombinant Pharmaceutical Protein Expression System of Henan,Xinxiang Medical University,Xinxiang 453003,China [3]pharmacy College,Xinxiang Medical University,Xinxiang 453003,China

出　　处：《Acta Biochimica et Biophysica Sinica》2020年第11期1285-1288,共4页生物化学与生物物理学报（英文版）

基　　金：the grants from the National Nat-ural Science Foundation of China(No.U1804168);the Plan of Scientific and Technological Innovation Team for University,Henan Province,China(No.18IRTSTHN027);the Key Science and Tech-nology Project of Henan(No.192102310149);the Key Sci-entific Research Project of Universities of Henan Province(No.18A350008).

摘　　要：Gene therapy is an effective strategy for treating several diseases,and safe and efficient vector systems are the basis for gene therapy applications.The non-viral episomal vectors are preferred as a gene transfer tool because they do not integrate into the host cell chromosome,which can avoid insertion mutation to prevent host immune response and potential tumorigenicity during disease treatment.In our previous study,we constructed an episomal vector driven by human cytomegalovirus major immediate-early(CMV)promoter and mediated by the characteristic motifs of scaffold/matrix attachment regions(S/MARs)[1].The vector contains only 387-bp MARs instead of the 2200-bp S/MARs in the original non-viral episomal vector pEPI,which showed the higher transgene efficiency and expression level[2].

关 键 词：MEDIATED diseases INSERTION 

分 类 号：Q78[生物学—分子生物学]