刀豆壳总黄酮提取工艺优化及其抗氧化活性研究  

Optimization of Extraction Technology and Its Antioxidant Activity of Total Flavonoids from Concanavalis Shell

作　　者：唐森 李璐彬 覃逸明[2,3] 吴国勇 张鹏 TANG Sen;LI Lu-bin;QIN Yi-ming;WU Guo-yong;ZHANG Peng(School of Food and Biochemical Engineering,GuangxiScience&Technology Normal University,Laibin 546199,Guangxi,China;Key Laboratory for Research and Development of Characteristic Yao Medicine Resources,Guangxi Science&Technology Normal University,Laibin 546199,Guangxi,China;Scientific Research Management Department,Guangxi Science&Technology Normal University,Laibin 546199,Guangxi,China)

机构地区：[1]广西科技师范学院食品与生化工程学院,广西来宾546199 [2]广西科技师范学院特色瑶药资源研究与开发重点实验室,广西来宾546199 [3]广西科技师范学院科研管理处,广西来宾546199

出　　处：《食品研究与开发》2021年第6期76-84,共9页Food Research and Development

基　　金：广西糖资源工程技术研究中心培育建设项目(桂科AD16450040);广西高校制糖工程综合技术重点实验室培育建设项目(桂教科研〔2016〕6号);2018-2020年广西本科高校食品科学与工程特色专业及实验实训教学基地(中心)建设项目(桂教高教[2018]52号);广西金秀桐木镇瑶医瑶药创新小镇培育建设项目(桂科高字[2018]299号)。

摘　　要：以刀豆壳的总黄酮提取量为评价指标,使用乙醇为提取剂,微波辅助提取刀豆壳总黄酮,分别考察乙醇体积分数(%)、液料比(mL/g)、微波时间(min)、微波功率(W)对刀豆壳总黄酮提取量的影响,通过响应面法优化提取工艺并研究提取的刀豆壳总黄酮的抗氧化活性。经试验得出当乙醇体积分数为50%,液料比为20:1(mL/g),微波时间为5.6 min,微波功率为600 W,总黄酮提取量为2.330 mg/g;刀豆壳总黄酮对羟自由基清除作用明显,其质量浓度与抗氧化活性有一定的量效关系。The total flavonoids extracted from the soybean hull was used as the evaluation index,ethanol was used as the extractant,and flavonoids extracted from the Concanavalis shell by microwave,the ethanol volume fraction(%),liquid ratio(mL/g),microwave time(min),microwave power(W)on the content of Concanavalis shell flavonoids was investigated by the response surface method to optimize the process and the antioxidant activity of the Concanavalis shell flavonoids were studied.The test resulted that when the volume score of ethanol was 50%,the liquid ratio was 20∶1(mL/g),the microwave time was 5.6 min,the microwave power was 600 W,total the flavonoid content was 2.330 mg/g;Concanavalis shell flavonoid hydroxyl removal effect was obvious,its mass concentration and antioxidant activity had a certain quantitative effect relationship.

关 键 词：刀豆壳 提取 工艺优化 黄酮 响应面 抗氧化活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]