绿茶多酚改善社会挫败模型小鼠的抑郁行为研究  

Research on green tea polyphenols improved depressive behavior of social frustration depression model mice

作　　者：刘志强[1] 邹永周 何敏[1] 杨平[1] 屈新辉[1] LIU Zhiqiang;ZOU Yongzhou;HE Min;YANG Ping;QU Xinhui(Department of Neurology,Second Department of Jiangxi Provincial People′s Hospital/People′s Hospital Affiliated to Nanchang University,Nanchang,Jiangxi 330000,China;Department of Neurology,First People′s Hospital of Jingdezhen City,Jingdezhen,Jiangxi 333000,China)

机构地区：[1]江西省人民医院/南昌大学附属人民医院二部神经内科,南昌330000 [2]江西省景德镇市第一人民医院神经内科,333000

出　　处：《重庆医学》2021年第5期741-745,共5页Chongqing medicine

基　　金：江西省卫生健康委科技计划资助项目(20203027)。

摘　　要：目的探讨绿茶多酚对社会挫败模型小鼠的抗抑郁作用及相关机制。方法用C57BL/6小黑鼠建立社会挫败模型;应用强迫游泳行为学方法评估绿茶多酚对模型小鼠抑郁行为的影响;应用水迷宫行为学方法评估茶多酚对模型小鼠认知功能的影响;取各组小鼠海马组织,应用Western blot技术测定磷酸化环腺苷效应元件结合蛋白(p-CREB)及脑源性神经生长因子(BDNF)的表达。结果绿茶多酚可以改善社会挫败模型小鼠强迫游泳不动时间,且与浓度相关;绿茶多酚可以减少社会挫败模型小鼠水迷宫潜伏期时间,且与浓度相关;海马区CREB-BNDF通路参与绿茶多酚抗抑郁作用的调节。结论绿茶多酚可以通过海马区CREB-BDNF通路改善社会挫败抑郁模型鼠的行为。Objective To investigate the antidepressant effect of green tea polyphenols on social frustration depression model mice and related mechanisms.Methods C57BL/6 black mice was used to establish the social frustration model;forced swimming behavior method was used to evaluate the effect of green tea polyphenols on depression behavior in model mice;water maze behavior method was used to evaluate the effects of green tea polyphenols on cognitive function of model mice;Hippocampus of each group were took,and the expression levels of phosphorylated cyclic adenosine phosphate response element binding protein(CREB)and brain-derived nerve growth factor(BDNF)were detected by using Western blot.Results Green tea polyphenols could improve the time of forced swimming immobility in model mice,and it was related to the concentration of green tea polyphenons.Green tea polyphenols could reduce the incubation period of the water maze in model mice,and it was related to the concentration of green tea polyphenons.CREB-BDNF pathway in the hippocampus of the mice was involved in the regulation of antidepressant effect of green tea polyphenols.Conclusion Green tea polyphenols can improve the behavior of social frustration depression model mice through the CREB-BDNF pathway in the hippocampus.

关 键 词：绿茶多酚 强迫游泳 MORRIS水迷宫 抑郁症 社会挫败 神经生长因子 

分 类 号：R-332[医药卫生] R332.81