蜂胶提取物对诺如病毒的体外抑制作用研究  

Inhibitory Effect of Propolis Extract on Norovirus in Vitro

作　　者：唐梦旋 陈莉莉[2] 廖宁波 陈江[3] 程东庆 吴国平 TANG Mengxuan;CHEN Lili;LIAO Ningbo;CHEN Jiang;CHENG Dongqing;WU Guoping(College of Food Science and Engineering,Jiangxi Agricultural University, Nanchang 330000, China;Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China;College of Medical Technology, Zhejiang Chinese Medical University, Hangzhou 310053, China)

机构地区：[1]江西农业大学食品科学与工程学院,江西南昌330000 [2]浙江省疾病预防控制中心,浙江杭州310051 [3]浙江中医药大学医学技术学院,浙江杭州310053

出　　处：《食品科学技术学报》2021年第2期40-47,共8页Journal of Food Science and Technology

基　　金：浙江省公益性技术应用研究项目(#LGN20C200004);江西省青年人才培养项目(29202000031)。

摘　　要：为研究蜂胶提取物对人源诺如病毒的体外抑制作用,采用小鼠诺如病毒和噬菌体MS2病毒为替代病毒,研究蜂胶水提物和醇提物随浓度及时间变化对小鼠诺如病毒和噬菌体MS2病毒的抑制作用及其机制。浓度相关性实验表明:随着蜂胶醇提物浓度增加,小鼠诺如病毒和MS2病毒滴度呈显著下降趋势,当醇提物质量浓度达到500μg/mL时,小鼠诺如病毒和MS2病毒滴度分别下降了6.93 lgPFU/mL和3.75 lgPFU/mL。时间相关性实验表明:将质量浓度为250μg/mL蜂胶醇提物与小鼠诺如病毒和MS2病毒培养60 min,前40 min小鼠诺如病毒和MS2病毒滴度分别下降4.18 lgPFU/mL和3.30 lgPFU/mL,随后下降趋势减缓,在60 min时,小鼠诺如病毒和MS2病毒的总滴度分别下降了4.48 lgPFU/mL和3.67 lgPFU/mL。机制研究表明:蜂胶醇提物可能与宿主细胞(受体)结合,或直接与病毒颗粒结合,使病毒衣壳蛋白变性,阻止病毒进入宿主细胞;透射电镜图像进一步证实了蜂胶醇提物直接作用病毒导致其膨胀破裂。最后,经蜂胶醇提物处理后,人为污染病毒的蔬菜汁和果汁中病毒的滴度显著降低。研究结果将有可能为果蔬在鲜榨过程中,挑选合适的添加剂提供更多的选择。In order to study the inhibitory effect of propolis extract on human norovirus in vitro,murine norovirus and phage MS2 virus were used as alternative viruses to study the inhibitory effect and mechanism of propolis aqueous extract and ethanol extract at different concentrations and time.Concentration-dependent experiments showed that the titers of murine norovirus and MS2 viruses decreased significantly with the increasing concentration of propolis ethanol extract.When the mass concentration of propolis ethanol extract reached 500μg/mL,the titers of murine norovirus and MS2 viruses decreased by 6.93 lgPFU/mL and 3.75 lgPFU/mL,respectively.The time-correlation experiment showed that during murine norovirus and MS2 for 60 min culturing with 250μg/mL propolis ethanol extract,the virus titers of murine norovirus and MS2 decreased by 4.18 lgPFU/mL and 3.30 lgPFU/mL in the first 40 min,and then slowed down.After culture for 60 min,the total titers of murine norovirus and MS2 decreased by 4.48 lgPFU/mL and 3.67 lgPFU/mL,respectively.Mechanism studies had shown that propolis ethanol extract may bind to host cells(receptors)or directly bind to virus particles to denature viral capsid proteins and prevent viruses from entering host cells.Images taken by transmission electron microscope further confirmed that propoils ethanol extract directly acted on the virus and triggered expansion and rupture.Finally,after propolis ethanol extract treatment,the titer of virus in the vegetable and fruit juices contaminated with man-made virus was significantly reduced.The results of this study might provide more options for selecting suitable additives in fresh pressing process of fruits and vegetables.

关 键 词：蜂胶提取物 人源诺如病毒 小鼠诺如病毒 噬菌体 天然添加剂 

分 类 号：TS202.3[轻工技术与工程—食品科学] TS255.3[轻工技术与工程—食品科学与工程]