Cytotoxic effects of docetaxel as a candidate drug of drug-eluting stent on human umbilical vein endothelial cells and the signaling pathway of cell migration inhibition,adhesion delay and shape change  

作　　者：Tingzhang Hu Chun Yang Meiling Fu Jiali Yang Rolin Du Xiaolin Ran Tieying Yin Guixue Wang 

机构地区：[1]Key Laboratory for Biorheological Science and Technology of Ministry of Education,State and Local Joint Engineering Laboratory for Vascular Implants,Bioengineering College of Chongqing University,Chongqing 400030,China

出　　处：《Regenerative Biomaterials》2017年第3期167-178,共12页再生生物材料（英文版）

基　　金：The study was supported by grants from the National Natural Science Foundation of China(11332003,31370949 and 81400329);the National Key Technology R&D Program of China(2016YFC1102305 and 2012BAI18B02);the Chongqing Graduate Student Research Innovation Project(CYS14023);the Fundamental Research Funds for the Central Universities(106112016CDJXZ238802);as well as the support from the Chongqing Engineering Laboratory in Vascular Implants and the Public Experiment Center of State Bioindustrial Base(Chongqing),China.

摘　　要：Docetaxel(DTX),a paclitaxel analogue,can efficiently inhibit proliferation of vascular smooth muscle cells and has broadly been used as an antiangiogenesis drug.However,as a candidate drug of drug-eluting stent,the effects of DTX on human umbilical vein endothelial cells(HUVECs)are still not well understood.Herein,we investigated the effects of DTX on proliferation,apoptosis,adhesion,migration and morphology of HUVECs in vitro.We found that DTX had the cytostatic and cytotoxic effects at low and high concentrations,respectively.DTX could inhibit the proliferation and migration of HUVECs,induce HUVECs apoptosis,delay HUVECs adhesion and decrease spreading area and aspect ratio of individual cells.The signaling pathway that DTX led to the migration inhibition,adhesion delay and shape change of HUVECs is the VE-cadherin mediated integrin b1/FAK/ROCK signaling pathway.The study will provide a theoretical basis for the clinical application of DTX.

关 键 词：cytotoxic effects DOCETAXEL endothelial cells signaling pathway 

分 类 号：R73[医药卫生—肿瘤]