Osteoblast behaviors on titania nanotube and mesopore layers  

作　　者：Yan Zhang Rong Luo Jing Tan Jianxin Wang Xiong Lu Shuxin Qu Jie Weng Bo Feng 

机构地区：[1]Key Laboratory of Advanced Technologies of Materials,Ministry of Education,School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,PR China

出　　处：《Regenerative Biomaterials》2017年第2期81-87,共7页再生生物材料（英文版）

基　　金：This work was supported by the National Basic Research Program of China(973 Program,2012CB933600);Applied Basic Research Programs of Sichuan Province,China(2015JY0036).

摘　　要：Titania nanotubes and mesopores with different diameter sizes were prepared by electrochemical oxidation of titanium.The responses of osteoblastic cells isolated from Sprague–Dawley rats to the nanotube and mesopore layers were investigated in sequential events of cell adhesion,morphology,actin cytoskeleton,proliferation,differentiation,and mineralization.Nano-structural features,especially diameters of the nanotubes and mesopores,obviously influenced on cell behaviors in the sequential events.The cells showed better proliferation and differentiation abilities on the specimens with the nanotubes and mesopores than on flat titanium disk.Higher levers of calcium mineralization were observed on the nanotube and mesopore layers.The cells adhered much faster onto the nanotubes with about 170nm diameter and the mesopores with about 400nm diameter than onto flat titanium disk and 50nm nanotubes.There is an appropriate range of the tube/pore sizes,and in this present work,titania nantubes with 170nm diameter is the best for enhancing functions of osteoblasts.

关 键 词：NANOTUBE MESOPORE OSTEOBLAST adhesion PROLIFERATION MINERALIZATION 

分 类 号：TB3[一般工业技术—材料科学与工程]